Lipids and membranes in the organic matrix of urinary calcific crystals and stones

S. R. Khan; F. Atmani; P. Glenton; Z. C. Hou; D. R. Talham; M. Khurshid

doi:10.1007/s002239900140

Lipids and membranes in the organic matrix of urinary calcific crystals and stones

S. R. Khan, F. Atmani, P. Glenton, Z. C. Hou, D. R. Talham, M. Khurshid

Research output: Contribution to journal › Article › peer-review

55 Citations (Scopus)

Abstract

The ultrastructure of the organic matrix of demineralized urinary stones was examined by standard transmission and scanning electron microscopy as well as after malachite green-glutaraldehyde fixation. Crystal ghosts of both calcium oxalate and calcium phosphate were made of amorphous material and were dispersed in a matrix containing amorphous, fibrillar, and membranous substances. Malachite green positive material was seen to be associated with the ghosts, as well as with the membranous and fibrillar components of the organic matrix. Calcium oxalate and calcium-phosphate crystals, induced in human urine in vitro were also found to be associated with an organic matrix containing lipids and proteins. It is suggested that the intimate association between crystals and lipids is a result of the involvement of cellular membranes in the nucleation of these crystals.

Original language	English
Pages (from-to)	357-365
Number of pages	9
Journal	Calcified Tissue International
Volume	59
Issue number	5
DOIs	https://doi.org/10.1007/s002239900140
Publication status	Published - Nov 1996
Externally published	Yes

Keywords

Calcium oxalate
Calcium phosphate
Lipids
Membranes
Nephrolithiasis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s002239900140

Cite this

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title = "Lipids and membranes in the organic matrix of urinary calcific crystals and stones",

abstract = "The ultrastructure of the organic matrix of demineralized urinary stones was examined by standard transmission and scanning electron microscopy as well as after malachite green-glutaraldehyde fixation. Crystal ghosts of both calcium oxalate and calcium phosphate were made of amorphous material and were dispersed in a matrix containing amorphous, fibrillar, and membranous substances. Malachite green positive material was seen to be associated with the ghosts, as well as with the membranous and fibrillar components of the organic matrix. Calcium oxalate and calcium-phosphate crystals, induced in human urine in vitro were also found to be associated with an organic matrix containing lipids and proteins. It is suggested that the intimate association between crystals and lipids is a result of the involvement of cellular membranes in the nucleation of these crystals.",

keywords = "Calcium oxalate, Calcium phosphate, Lipids, Membranes, Nephrolithiasis",

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T1 - Lipids and membranes in the organic matrix of urinary calcific crystals and stones

AU - Khan, S. R.

AU - Atmani, F.

AU - Glenton, P.

AU - Hou, Z. C.

AU - Talham, D. R.

AU - Khurshid, M.

PY - 1996/11

Y1 - 1996/11

N2 - The ultrastructure of the organic matrix of demineralized urinary stones was examined by standard transmission and scanning electron microscopy as well as after malachite green-glutaraldehyde fixation. Crystal ghosts of both calcium oxalate and calcium phosphate were made of amorphous material and were dispersed in a matrix containing amorphous, fibrillar, and membranous substances. Malachite green positive material was seen to be associated with the ghosts, as well as with the membranous and fibrillar components of the organic matrix. Calcium oxalate and calcium-phosphate crystals, induced in human urine in vitro were also found to be associated with an organic matrix containing lipids and proteins. It is suggested that the intimate association between crystals and lipids is a result of the involvement of cellular membranes in the nucleation of these crystals.

AB - The ultrastructure of the organic matrix of demineralized urinary stones was examined by standard transmission and scanning electron microscopy as well as after malachite green-glutaraldehyde fixation. Crystal ghosts of both calcium oxalate and calcium phosphate were made of amorphous material and were dispersed in a matrix containing amorphous, fibrillar, and membranous substances. Malachite green positive material was seen to be associated with the ghosts, as well as with the membranous and fibrillar components of the organic matrix. Calcium oxalate and calcium-phosphate crystals, induced in human urine in vitro were also found to be associated with an organic matrix containing lipids and proteins. It is suggested that the intimate association between crystals and lipids is a result of the involvement of cellular membranes in the nucleation of these crystals.

KW - Calcium oxalate

KW - Calcium phosphate

KW - Lipids

KW - Membranes

KW - Nephrolithiasis

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DO - 10.1007/s002239900140

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EP - 365

JO - Calcified Tissue International

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Lipids and membranes in the organic matrix of urinary calcific crystals and stones

Abstract

Keywords

UN SDGs

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