@article{0d9a157435854f4888e84f21dae35c55,
title = "Longitudinal changes of one-carbon metabolites and amino acid concentrations during pregnancy in the women first maternal nutrition trial",
abstract = "Background: Maternal dietary restriction and supplementation of one-carbon (1C) metabolites can impact offspring growth and DNA methylation. However, longitudinal research of 1C metabolite and amino acid (AA) concentrations over the reproductive cycle of human pregnancy is limited. Objective: To investigate longitudinal 1C metabolite and AA concentrations prior to and during pregnancy and the effects of a small-quantity lipid-based nutrition supplement (LNS) containing >20 micronutrients and prepregnancy BMI (ppBMI). Methods: This study was an ancillary study of the Women First Trial (NCT01883193, clinicaltrials.gov) focused on a subset of Guatemalan women (n = 134), 49% of whom entered pregnancy with a BMI ≥25 kg/m2. Ninety-five women received LNS during pregnancy (+LNS group), while the remainder did not (-LNS group). A subset of women from the Pakistan study site (n = 179) were used as a replication cohort, 124 of whom received LNS. Maternal blood was longitudinally collected on dried blood spot (DBS) cards at preconception, and at 12 and 34 wk gestation. A targeted metabolomics assay was performed on DBS samples at each time point using LC-MS/MS. Longitudinal analyses were performed using linear mixed modeling to investigate the influence of time, LNS, and ppBMI. Results: Concentrations of 23 of 27 metabolites, including betaine, choline, and serine, changed from preconception across gestation after application of a Bonferroni multiple testing correction (P < 0.00185). Sixteen of those metabolites showed similar changes in the replication cohort. Asymmetric and symmetric dimethylarginine were decreased by LNS in the participants from Guatemala. Only tyrosine was statistically associated with ppBMI at both study sites. Conclusions: Time influenced most 1C metabolite and AA concentrations with a high degree of similarity between the 2 diverse study populations. These patterns were not significantly altered by LNS consumption or ppBMI. Future investigations will focus on 1C metabolite changes associated with infant outcomes, including DNA methylation.",
keywords = "Amino acids, BMI, Malnutrition, Obesity, One-carbon metabolism, Preconception, Pregnancy, Supplementation, Triple nutrition burden",
author = "Gilley, {Stephanie P.} and Weaver, {Nicholas E.} and Sticca, {Evan L.} and Purevsuren Jambal and Alexandra Palacios and Kerns, {Mattie E.} and Pratibha Anand and Kemp, {Jennifer F.} and Westcott, {Jamie E.} and Lester Figueroa and Garces, {Ana Lucia} and Ali, {Sumera A.} and Omrana Pasha and Sarah Saleem and Hambidge, {K. Michael} and Hendricks, {Audrey E.} and Krebs, {Nancy F.} and Borengasser, {Sarah J.}",
note = "Funding Information: Copyright {\textcopyright}C The Author(s) 2019. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. Manuscript received June 6, 2019. Initial review completed October 9, 2019. Revision accepted November 15, 2019. Published online November 18, 2019. NFK and SJB contributed equally to this work. Supported by the Bill & Melinda Gates Foundation OPP1055867 (NFK and KMH); the Eunice Kennedy Shriver National Institute of Child Health and Human Development and Office of Dietary Supplements U10 HD076474 (KMH and NFK); and the National Institute of Diabetes and Digestive and Kidney Diseases K01 DK109077 (SJB). This publication was also made possible by the University of Florida{\textquoteright}s Southeast Center for Integrated Metabolomics through grant number U24 DK097209 from the National Institute of Health{\textquoteright}s Common Fund metabolomics program. The funding bodies had no role in the design of the present study, nor with collection, analysis, and interpretation of data or in writing the manuscript. Author disclosures: The authors report no conflicts of interest. Availability of data and material: The metabolomics datasets will be deposited to the University of California San Diego Metabolomics Workbench (www.metabolomicsworkbench.org). Supplemental Table 1 and Supplemental Figures 1 and 2 are available from the “Supplementary data” link in the online posting of the article and from the same link in the online table of contents at https://academic.oup.com/cdn/. Address correspondence to SB (e-mail: sarah.borengasser@cuanschutz.edu). Abbreviations used: AA, amino acid; ADMA, asymmetric dimethylarginine; DBS, dried blood spot; LME, linear mixed effect; LNS, small-quantity lipid-based nutrition supplement; ppBMI, prepregnancy BMI; SDMA, symmetric dimethylarginine; SES, socioeconomic status; 1C, one carbon. Publisher Copyright: {\textcopyright} 2019 The Author(s).",
year = "2020",
doi = "10.1093/CDN/NZZ132",
language = "English",
volume = "4",
journal = "Current Developments in Nutrition",
issn = "2475-2991",
publisher = "Oxford University Press",
number = "1",
}
