TY - JOUR
T1 - Mechanisms underlying the antispasmodic and bronchodilatory properties of Terminalia bellerica fruit
AU - Gilani, Anwarul Hassan
AU - Khan, Arif ullah
AU - Ali, Tuba
AU - Ajmal, Saad
N1 - Funding Information:
This study was supported by funds made available by the Higher Education Commission of Pakistan under the scheme of Distinguished National Professor Research Allowance.
PY - 2008/3/28
Y1 - 2008/3/28
N2 - Aim of the study: The present investigation was carried out to provide the pharmacological basis for the medicinal use of Terminalia bellerica in hyperactive gastrointestinal and respiratory disorders. Materials and methods: Crude extract of Terminalia bellerica fruit (Tb.Cr) was studied in in vitro and in vivo. Results: Tb.Cr caused relaxation of spontaneous contractions in isolated rabbit jejunum at 0.1-3.0 mg/mL. Tb.Cr inhibited the carbachol (CCh, 1 μM) and K+ (80 mM)-induced contractions in a pattern similar to that of dicyclomine, but different from nifedipine and atropine. Tb.Cr shifted the Ca++ concentration-response curves to right, like nifedipine and dicyclomine. In guinea-pig ileum, Tb.Cr produced rightward parallel shift of acetylcholine-curves, followed by non-parallel shift at higher concentration with the suppression of maximum response, similar to dicyclomine, but different from nifedipine and atropine. Tb.Cr exhibited protective effect against castor oil-induced diarrhea and carbachol-mediated bronchoconstriction in rodents. In guinea-pig trachea, Tb.Cr relaxed the CCh-induced contractions, shifted CCh-curves to right and inhibited the contractions of K+. Anticholinergic effect was distributed both in organic and aqueous fractions, while CCB was present in the aqueous fraction. Conclusions: These results indicate that Terminalia bellerica fruit possess a combination of anticholinergic and Ca++ antagonist effects, which explain its folkloric use in the colic, diarrhea and asthma.
AB - Aim of the study: The present investigation was carried out to provide the pharmacological basis for the medicinal use of Terminalia bellerica in hyperactive gastrointestinal and respiratory disorders. Materials and methods: Crude extract of Terminalia bellerica fruit (Tb.Cr) was studied in in vitro and in vivo. Results: Tb.Cr caused relaxation of spontaneous contractions in isolated rabbit jejunum at 0.1-3.0 mg/mL. Tb.Cr inhibited the carbachol (CCh, 1 μM) and K+ (80 mM)-induced contractions in a pattern similar to that of dicyclomine, but different from nifedipine and atropine. Tb.Cr shifted the Ca++ concentration-response curves to right, like nifedipine and dicyclomine. In guinea-pig ileum, Tb.Cr produced rightward parallel shift of acetylcholine-curves, followed by non-parallel shift at higher concentration with the suppression of maximum response, similar to dicyclomine, but different from nifedipine and atropine. Tb.Cr exhibited protective effect against castor oil-induced diarrhea and carbachol-mediated bronchoconstriction in rodents. In guinea-pig trachea, Tb.Cr relaxed the CCh-induced contractions, shifted CCh-curves to right and inhibited the contractions of K+. Anticholinergic effect was distributed both in organic and aqueous fractions, while CCB was present in the aqueous fraction. Conclusions: These results indicate that Terminalia bellerica fruit possess a combination of anticholinergic and Ca++ antagonist effects, which explain its folkloric use in the colic, diarrhea and asthma.
KW - Anticholinergic
KW - Ca antagonist
KW - Gastrointestinal and respiratory disorders
KW - Terminalia bellerica fruit
UR - http://www.scopus.com/inward/record.url?scp=40249086505&partnerID=8YFLogxK
U2 - 10.1016/j.jep.2008.01.006
DO - 10.1016/j.jep.2008.01.006
M3 - Article
C2 - 18289813
AN - SCOPUS:40249086505
SN - 0378-8741
VL - 116
SP - 528
EP - 538
JO - Journal of Ethnopharmacology
JF - Journal of Ethnopharmacology
IS - 3
ER -
