Microwave-stimulated immunogold silver staining

P. Jackson; E. N. Lalani; J. Boutsen

doi:10.1007/BF01002729

Microwave-stimulated immunogold silver staining

P. Jackson, E. N. Lalani, J. Boutsen

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

The effect of microwave-generated energy for the demonstration of immunoglobulins in periodate lysine paraformaldehyde dichromate fixed paraffin sections of human reactive tonsil using the immunogold silver staining technique has been evaluated. Results of the study show that microwave stimulation permits incubation times of both the primary antibody and the immunogold reagent to be greatly reduced. Specific staining of equal intensity to that produced using the longer standard procedure together with minimal background staining is achieved.

Original language	English
Pages (from-to)	353-358
Number of pages	6
Journal	Journal of Molecular Histology
Volume	20
Issue number	6-7
DOIs	https://doi.org/10.1007/BF01002729
Publication status	Published - Jun 1988
Externally published	Yes

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10.1007/BF01002729

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title = "Microwave-stimulated immunogold silver staining",

abstract = "The effect of microwave-generated energy for the demonstration of immunoglobulins in periodate lysine paraformaldehyde dichromate fixed paraffin sections of human reactive tonsil using the immunogold silver staining technique has been evaluated. Results of the study show that microwave stimulation permits incubation times of both the primary antibody and the immunogold reagent to be greatly reduced. Specific staining of equal intensity to that produced using the longer standard procedure together with minimal background staining is achieved.",

author = "P. Jackson and Lalani, {E. N.} and J. Boutsen",

year = "1988",

month = jun,

doi = "10.1007/BF01002729",

language = "English",

volume = "20",

pages = "353--358",

journal = "Journal of Molecular Histology",

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AU - Jackson, P.

AU - Lalani, E. N.

AU - Boutsen, J.

PY - 1988/6

Y1 - 1988/6

N2 - The effect of microwave-generated energy for the demonstration of immunoglobulins in periodate lysine paraformaldehyde dichromate fixed paraffin sections of human reactive tonsil using the immunogold silver staining technique has been evaluated. Results of the study show that microwave stimulation permits incubation times of both the primary antibody and the immunogold reagent to be greatly reduced. Specific staining of equal intensity to that produced using the longer standard procedure together with minimal background staining is achieved.

AB - The effect of microwave-generated energy for the demonstration of immunoglobulins in periodate lysine paraformaldehyde dichromate fixed paraffin sections of human reactive tonsil using the immunogold silver staining technique has been evaluated. Results of the study show that microwave stimulation permits incubation times of both the primary antibody and the immunogold reagent to be greatly reduced. Specific staining of equal intensity to that produced using the longer standard procedure together with minimal background staining is achieved.

UR - http://www.scopus.com/inward/record.url?scp=0023807995&partnerID=8YFLogxK

U2 - 10.1007/BF01002729

DO - 10.1007/BF01002729

M3 - Article

C2 - 2464567

AN - SCOPUS:0023807995

SN - 0018-2214

VL - 20

SP - 353

EP - 358

JO - Journal of Molecular Histology

JF - Journal of Molecular Histology

IS - 6-7

ER -

Microwave-stimulated immunogold silver staining

Abstract

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