Mimicking herpes simplex virus 1 and herpes simplex virus 2 mucosal behavior in a well-characterized human genital organ culture

Lennert Steukers; Steven Weyers; Xiaoyun Yang; Annelies P. Vandekerckhove; Sarah Glorieux; Maria Cornelissen; Wim Van Den Broeck; Marleen Temmerman; Hans J. Nauwynck

doi:10.1093/infdis/jiu036

Mimicking herpes simplex virus 1 and herpes simplex virus 2 mucosal behavior in a well-characterized human genital organ culture

Lennert Steukers
, Steven Weyers
, Xiaoyun Yang
, Annelies P. Vandekerckhove
, Sarah Glorieux
, Maria Cornelissen
, Wim Van Den Broeck
, Marleen Temmerman
, Hans J. Nauwynck

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

We developed and morphologically characterized a human genital mucosa explant model (endocervix and ectocervix/vagina) to mimic genital herpes infections caused by herpes simplex virus types 1 (HSV-1) and 2 (HSV-2). Subsequent analysis of HSV entry receptor expression throughout the menstrual cycle in genital tissues was performed, and the evolution of HSV-1/-2 mucosal spread over time was assessed. Nectin-1 and -2 were expressed in all tissues during the entire menstrual cycle. Herpesvirus entry mediator expression was limited mainly to some connective tissue cells. Both HSV-1 and HSV-2 exhibited a plaque-wise mucosal spread across the basement membrane and induced prominent epithelial syncytia.

Original language	English (UK)
Pages (from-to)	209-213
Number of pages	5
Journal	Journal of Infectious Diseases
Volume	210
Issue number	2
DOIs	https://doi.org/10.1093/infdis/jiu036
Publication status	Published - 15 Jul 2014
Externally published	Yes

Keywords

HVEM

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1093/infdis/jiu036

Cite this

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title = "Mimicking herpes simplex virus 1 and herpes simplex virus 2 mucosal behavior in a well-characterized human genital organ culture",

abstract = "We developed and morphologically characterized a human genital mucosa explant model (endocervix and ectocervix/vagina) to mimic genital herpes infections caused by herpes simplex virus types 1 (HSV-1) and 2 (HSV-2). Subsequent analysis of HSV entry receptor expression throughout the menstrual cycle in genital tissues was performed, and the evolution of HSV-1/-2 mucosal spread over time was assessed. Nectin-1 and -2 were expressed in all tissues during the entire menstrual cycle. Herpesvirus entry mediator expression was limited mainly to some connective tissue cells. Both HSV-1 and HSV-2 exhibited a plaque-wise mucosal spread across the basement membrane and induced prominent epithelial syncytia.",

keywords = "HVEM",

author = "Lennert Steukers and Steven Weyers and Xiaoyun Yang and Vandekerckhove, \{Annelies P.\} and Sarah Glorieux and Maria Cornelissen and \{Van Den Broeck\}, Wim and Marleen Temmerman and Nauwynck, \{Hans J.\}",

note = "Funding Information: Financial support. This work was supported by the Agency for Innovation by Science and Technology (PhD grants SB 091197 and 093197) and by a Concerted Research Action of Ghent University. Lennert Steukers and co-workers are members of the BELVIR consortium (IAP, phase VII) sponsored by Belgian Science Policy Office (BELSPO). Potential conflicts of interest. All authors: No reported conflicts.",

year = "2014",

month = jul,

day = "15",

doi = "10.1093/infdis/jiu036",

language = "English (UK)",

volume = "210",

pages = "209--213",

journal = "Journal of Infectious Diseases",

issn = "0022-1899",

publisher = "Oxford University Press",

number = "2",

}

TY - JOUR

T1 - Mimicking herpes simplex virus 1 and herpes simplex virus 2 mucosal behavior in a well-characterized human genital organ culture

AU - Steukers, Lennert

AU - Weyers, Steven

AU - Yang, Xiaoyun

AU - Vandekerckhove, Annelies P.

AU - Glorieux, Sarah

AU - Cornelissen, Maria

AU - Van Den Broeck, Wim

AU - Temmerman, Marleen

AU - Nauwynck, Hans J.

N1 - Funding Information: Financial support. This work was supported by the Agency for Innovation by Science and Technology (PhD grants SB 091197 and 093197) and by a Concerted Research Action of Ghent University. Lennert Steukers and co-workers are members of the BELVIR consortium (IAP, phase VII) sponsored by Belgian Science Policy Office (BELSPO). Potential conflicts of interest. All authors: No reported conflicts.

PY - 2014/7/15

Y1 - 2014/7/15

N2 - We developed and morphologically characterized a human genital mucosa explant model (endocervix and ectocervix/vagina) to mimic genital herpes infections caused by herpes simplex virus types 1 (HSV-1) and 2 (HSV-2). Subsequent analysis of HSV entry receptor expression throughout the menstrual cycle in genital tissues was performed, and the evolution of HSV-1/-2 mucosal spread over time was assessed. Nectin-1 and -2 were expressed in all tissues during the entire menstrual cycle. Herpesvirus entry mediator expression was limited mainly to some connective tissue cells. Both HSV-1 and HSV-2 exhibited a plaque-wise mucosal spread across the basement membrane and induced prominent epithelial syncytia.

AB - We developed and morphologically characterized a human genital mucosa explant model (endocervix and ectocervix/vagina) to mimic genital herpes infections caused by herpes simplex virus types 1 (HSV-1) and 2 (HSV-2). Subsequent analysis of HSV entry receptor expression throughout the menstrual cycle in genital tissues was performed, and the evolution of HSV-1/-2 mucosal spread over time was assessed. Nectin-1 and -2 were expressed in all tissues during the entire menstrual cycle. Herpesvirus entry mediator expression was limited mainly to some connective tissue cells. Both HSV-1 and HSV-2 exhibited a plaque-wise mucosal spread across the basement membrane and induced prominent epithelial syncytia.

KW - HVEM

UR - https://www.scopus.com/pages/publications/84904022370

U2 - 10.1093/infdis/jiu036

DO - 10.1093/infdis/jiu036

M3 - Article

C2 - 24436451

AN - SCOPUS:84904022370

SN - 0022-1899

VL - 210

SP - 209

EP - 213

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

IS - 2

ER -

Mimicking herpes simplex virus 1 and herpes simplex virus 2 mucosal behavior in a well-characterized human genital organ culture

Abstract

Keywords

UN SDGs

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