TY - JOUR
T1 - Molecular analysis of household transmission of Giardia lamblia in a region of high endemicity in Peru
AU - Cooper, Margarethe A.
AU - Sterling, Charles R.
AU - Gilman, Robert H.
AU - Cama, Vitaliano
AU - Ortega, Ynes
AU - Adam, Rodney D.
N1 - Funding Information:
Financial support: National Institutes of Health (NIH) Minority International Research Training program (grant 5 T37 TW00036-09 to M.A.C.) and Achievement Rewards for College Scientists (to M.A.C). The project was also funded in part by grant UDSA-ARZT-136034-H-02-124 (to C.R.S.).
PY - 2010/12/1
Y1 - 2010/12/1
N2 - Background. Giardia lamblia is ubiquitous in multiple communities of nonindustrialized nations. Genotypes A1, A2, and B (Nash groups 1, 2, and 3, respectively) are found in humans, whereas genotypes C and D are typically found in dogs. However, genotypes A and B have occasionally been identified in dogs. Methods. Fecal Giardia isolates from 22 families and their dogs, living in Pampas de San Juan, were collected over 7 weeks in 2002 and 6 weeks in 2003. Samples were genotyped, followed by sequencing and haplotyping of many of these isolates by using loci on chromosomes 3 and 5. Results. Human infections were all caused by isolates of genotypes A2 and B. Human coinfections with genotypes A2 and B were common, and the reassortment pattern of different subtypes of A2 isolates supports prior observations that suggested recombination among genotype A2 isolates. All dogs had genotypes C and/or D, with one exception of a dog with a mixed B/D genotype infection. Conclusions. In a region of high endemicity where infected dogs and humans constantly commingle, different genotypes of Giardia are almost always found in dogs and humans, suggesting that zoonotic transmission is very uncommon.
AB - Background. Giardia lamblia is ubiquitous in multiple communities of nonindustrialized nations. Genotypes A1, A2, and B (Nash groups 1, 2, and 3, respectively) are found in humans, whereas genotypes C and D are typically found in dogs. However, genotypes A and B have occasionally been identified in dogs. Methods. Fecal Giardia isolates from 22 families and their dogs, living in Pampas de San Juan, were collected over 7 weeks in 2002 and 6 weeks in 2003. Samples were genotyped, followed by sequencing and haplotyping of many of these isolates by using loci on chromosomes 3 and 5. Results. Human infections were all caused by isolates of genotypes A2 and B. Human coinfections with genotypes A2 and B were common, and the reassortment pattern of different subtypes of A2 isolates supports prior observations that suggested recombination among genotype A2 isolates. All dogs had genotypes C and/or D, with one exception of a dog with a mixed B/D genotype infection. Conclusions. In a region of high endemicity where infected dogs and humans constantly commingle, different genotypes of Giardia are almost always found in dogs and humans, suggesting that zoonotic transmission is very uncommon.
UR - http://www.scopus.com/inward/record.url?scp=78649561799&partnerID=8YFLogxK
U2 - 10.1086/657142
DO - 10.1086/657142
M3 - Article
C2 - 20977340
AN - SCOPUS:78649561799
SN - 0022-1899
VL - 202
SP - 1713
EP - 1721
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 11
ER -
