TY - JOUR
T1 - Morphological study remains the mainstay in the diagnosis of small round blue cell tumours in childhood
AU - Khalid, Muneeza
AU - Nagi, A. H.
AU - Atif, Naila
AU - Din, Nasir U.
AU - Sheikh, Afzal
PY - 2013/4
Y1 - 2013/4
N2 - Aim: To study the importance of morphology in face of attractive new technology. Materials and methods: All tissue samples were fixed in formalin, embedded in paraffin and routinely stained with H and E, PAS for glycogen, silver for nerve fibers, PTAH for strap cells, CD 3, CD 20, and LCA for lymphoma. Reactions were preformed with appropriate positive and negative controls. Results: A total of 100 cases were included ,among these 29 were diagnosed as neuroblastoma,23 were Wilm's tumour, 14 were Non Hodgkin's lymphoma,9 were rhabdomyosarcoma, 8 were Ewing's sarcoma, 6 were medulloblastoma,5 were lymphoblastic lymphoma, 4 were retinoblastoma and 2 were Burkitt's lymphoma. Conclusion: Round blue cell tumours were more common in males in the first two decades of life. There is a significant association between presenting complaints and histological type of tumours. An association was also observed between histological type and morphological features. LCA CD 20 and CD 3s were helpful in confirming the diagnosis of lymphoma. In addition special stains such as PAS, silver and PTAH were very useful in differentiating small round blue cell tumours.
AB - Aim: To study the importance of morphology in face of attractive new technology. Materials and methods: All tissue samples were fixed in formalin, embedded in paraffin and routinely stained with H and E, PAS for glycogen, silver for nerve fibers, PTAH for strap cells, CD 3, CD 20, and LCA for lymphoma. Reactions were preformed with appropriate positive and negative controls. Results: A total of 100 cases were included ,among these 29 were diagnosed as neuroblastoma,23 were Wilm's tumour, 14 were Non Hodgkin's lymphoma,9 were rhabdomyosarcoma, 8 were Ewing's sarcoma, 6 were medulloblastoma,5 were lymphoblastic lymphoma, 4 were retinoblastoma and 2 were Burkitt's lymphoma. Conclusion: Round blue cell tumours were more common in males in the first two decades of life. There is a significant association between presenting complaints and histological type of tumours. An association was also observed between histological type and morphological features. LCA CD 20 and CD 3s were helpful in confirming the diagnosis of lymphoma. In addition special stains such as PAS, silver and PTAH were very useful in differentiating small round blue cell tumours.
KW - Rhabdomyosarcoma
KW - Small round blue cell tumours
KW - Wilm's tumour
UR - http://www.scopus.com/inward/record.url?scp=84885415186&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:84885415186
SN - 1996-7195
VL - 7
JO - Pakistan Journal of Medical and Health Sciences
JF - Pakistan Journal of Medical and Health Sciences
IS - 2
ER -