Mouse brain contains a high molecular weight nonfunctional protein with antigenic homology to dihydrofolate reductase

M. Perwaiz Iqbal; Sheldon P. Rothenberg; Charles Larosa

doi:10.1016/S0006-291X(86)80362-X

Mouse brain contains a high molecular weight nonfunctional protein with antigenic homology to dihydrofolate reductase

M. Perwaiz Iqbal, Sheldon P. Rothenberg, Charles Larosa

Research output: Contribution to journal › Article › peer-review

Abstract

The cytosol of brain tissue from mature BDF₁ mice contains very little dihydrofolate reductase activity but it does contain a high molecular weight nonfunctional protein which cross-reacts in a radioimmunoassay for the active enzyme. Liver cytosal contains less of this high molecular weight cross-reacting protein and more of the functional dihydrofolate reductase. The cytosol from kidney contains very little of the high molecular weight cross-reacting protein, 95% of the immunoreactive proteins being the functional form of dihydrofolate reductase. The modification of dihydrofolate reductase into a nonfunctional form may be an intrinsic property of some cells and this finding could explain the variability in measuring the activity of this enzyme in brain tissue of mature animals.

Original language	English (UK)
Pages (from-to)	258-262
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	141
Issue number	1
DOIs	https://doi.org/10.1016/S0006-291X(86)80362-X
Publication status	Published - 26 Nov 1986
Externally published	Yes

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10.1016/S0006-291X(86)80362-X

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title = "Mouse brain contains a high molecular weight nonfunctional protein with antigenic homology to dihydrofolate reductase",

abstract = "The cytosol of brain tissue from mature BDF1 mice contains very little dihydrofolate reductase activity but it does contain a high molecular weight nonfunctional protein which cross-reacts in a radioimmunoassay for the active enzyme. Liver cytosal contains less of this high molecular weight cross-reacting protein and more of the functional dihydrofolate reductase. The cytosol from kidney contains very little of the high molecular weight cross-reacting protein, 95\% of the immunoreactive proteins being the functional form of dihydrofolate reductase. The modification of dihydrofolate reductase into a nonfunctional form may be an intrinsic property of some cells and this finding could explain the variability in measuring the activity of this enzyme in brain tissue of mature animals.",

author = "Iqbal, \{M. Perwaiz\} and Rothenberg, \{Sheldon P.\} and Charles Larosa",

note = "Funding Information: 1 This work has been supported by a grant from the Veterans Administrat/on and the Kresevich Foundation of New York. 2 The present address of Dr. Iqbal is : Department of Biochemistry, The Aga Kahn Medical School, Karachi, Pakistan. 3 To whom reprint requests should be sent at the Brooklyn Veterans Administration Medical Center, 800 Poll\# Place, Brooklyn, New York 11209.",

year = "1986",

month = nov,

day = "26",

doi = "10.1016/S0006-291X(86)80362-X",

language = "English (UK)",

volume = "141",

pages = "258--262",

journal = "Biochemical and Biophysical Research Communications",

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T1 - Mouse brain contains a high molecular weight nonfunctional protein with antigenic homology to dihydrofolate reductase

AU - Iqbal, M. Perwaiz

AU - Rothenberg, Sheldon P.

AU - Larosa, Charles

N1 - Funding Information: 1 This work has been supported by a grant from the Veterans Administrat/on and the Kresevich Foundation of New York. 2 The present address of Dr. Iqbal is : Department of Biochemistry, The Aga Kahn Medical School, Karachi, Pakistan. 3 To whom reprint requests should be sent at the Brooklyn Veterans Administration Medical Center, 800 Poll# Place, Brooklyn, New York 11209.

PY - 1986/11/26

Y1 - 1986/11/26

N2 - The cytosol of brain tissue from mature BDF1 mice contains very little dihydrofolate reductase activity but it does contain a high molecular weight nonfunctional protein which cross-reacts in a radioimmunoassay for the active enzyme. Liver cytosal contains less of this high molecular weight cross-reacting protein and more of the functional dihydrofolate reductase. The cytosol from kidney contains very little of the high molecular weight cross-reacting protein, 95% of the immunoreactive proteins being the functional form of dihydrofolate reductase. The modification of dihydrofolate reductase into a nonfunctional form may be an intrinsic property of some cells and this finding could explain the variability in measuring the activity of this enzyme in brain tissue of mature animals.

AB - The cytosol of brain tissue from mature BDF1 mice contains very little dihydrofolate reductase activity but it does contain a high molecular weight nonfunctional protein which cross-reacts in a radioimmunoassay for the active enzyme. Liver cytosal contains less of this high molecular weight cross-reacting protein and more of the functional dihydrofolate reductase. The cytosol from kidney contains very little of the high molecular weight cross-reacting protein, 95% of the immunoreactive proteins being the functional form of dihydrofolate reductase. The modification of dihydrofolate reductase into a nonfunctional form may be an intrinsic property of some cells and this finding could explain the variability in measuring the activity of this enzyme in brain tissue of mature animals.

UR - https://www.scopus.com/pages/publications/0022898046

U2 - 10.1016/S0006-291X(86)80362-X

DO - 10.1016/S0006-291X(86)80362-X

M3 - Article

C2 - 3800999

AN - SCOPUS:0022898046

SN - 0006-291X

VL - 141

SP - 258

EP - 262

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Mouse brain contains a high molecular weight nonfunctional protein with antigenic homology to dihydrofolate reductase

Abstract

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