TY - JOUR
T1 - No change in calcium absorption in adult Pakistani population before and after vitamin D administration using strontium as surrogate
AU - Khan, A. H.
AU - Rohra, D. K.
AU - Saghir, S. A.
AU - Udani, S. K.
AU - Wood, R. J.
AU - Jabbar, A.
N1 - Funding Information:
The authors wish to acknowledge Higher Education Commission of Pakistan for funding the project. In addition, we wish to acknowledge the assistance of Gayle Petty, Director of the Nutrition Evaluation Laboratory at the Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University in Boston, MA and her staff for use of the direct current plasma emission spectroscope and technical assistance in analysis of the Sr samples.
PY - 2013/3
Y1 - 2013/3
N2 - Vitamin D, parathyroid hormone levels and calcium absorption was assessed before and after cholecalciferol using Strontium as a surrogate. Increase in 25OHD, lowering of iPTH with no effect on Sr absorption was seen, suggesting the possibility that maximal Ca absorption had already been achieved in these volunteers. Introduction: This paper discusses the determination of calcium (Ca) absorption, using strontium (Sr) as a surrogate, before and after a single IM injection of vitamin D3 (600,000 IU). Methods: Baseline serum 25-hydroxyvitamin D (25OHD), Sr, Ca, P, and intact parathyroid hormone (iPTH) were determined in 53 fasting volunteers, followed by administrating (PO) 0.03 mM (4.8 mg/kg) SrCl2 and collecting blood at 0.5, 1 and 4 h to determine the absorption (AUC0→t ) of Sr. Following the initial absorption test, volunteers received a single IM injection of 600,000 IU vitamin D3. Two months later, the fasting serum and the Sr absorption test were repeated, as described above. Results: The IM injection of vitamin D 3 caused a significant increase in fasting 25OHD (from 43.5 ± 19 to 66.1 ± 19.1 nmol/L (p < 0.001)) and a trend toward lower serum iPTH (from 59.8 ± 27.8 to 53 ± 31 ng/L). Fasting serum Ca and P remained unchanged. A higher 25OHD level failed (p = 0.32) to translate into a higher rate of Sr absorption. AUC0→4 h were almost identical before and after the IM injection of vitamin D3. Conclusion: A single vitamin D3 injection of 600,000 IU significantly increase mean 25OHD concentration and tended to lower iPTH concentrations in volunteers with initially low 25OHD status, suggesting to utilize this simple form of treatment to improve vitamin D status and to have a possible biological effect on Ca homeostasis. However, we found no obvious effect on Sr absorption, suggesting the possibility that maximal vitamin D-dependent Ca absorption had already been achieved in these volunteers at a lower vitamin D status.
AB - Vitamin D, parathyroid hormone levels and calcium absorption was assessed before and after cholecalciferol using Strontium as a surrogate. Increase in 25OHD, lowering of iPTH with no effect on Sr absorption was seen, suggesting the possibility that maximal Ca absorption had already been achieved in these volunteers. Introduction: This paper discusses the determination of calcium (Ca) absorption, using strontium (Sr) as a surrogate, before and after a single IM injection of vitamin D3 (600,000 IU). Methods: Baseline serum 25-hydroxyvitamin D (25OHD), Sr, Ca, P, and intact parathyroid hormone (iPTH) were determined in 53 fasting volunteers, followed by administrating (PO) 0.03 mM (4.8 mg/kg) SrCl2 and collecting blood at 0.5, 1 and 4 h to determine the absorption (AUC0→t ) of Sr. Following the initial absorption test, volunteers received a single IM injection of 600,000 IU vitamin D3. Two months later, the fasting serum and the Sr absorption test were repeated, as described above. Results: The IM injection of vitamin D 3 caused a significant increase in fasting 25OHD (from 43.5 ± 19 to 66.1 ± 19.1 nmol/L (p < 0.001)) and a trend toward lower serum iPTH (from 59.8 ± 27.8 to 53 ± 31 ng/L). Fasting serum Ca and P remained unchanged. A higher 25OHD level failed (p = 0.32) to translate into a higher rate of Sr absorption. AUC0→4 h were almost identical before and after the IM injection of vitamin D3. Conclusion: A single vitamin D3 injection of 600,000 IU significantly increase mean 25OHD concentration and tended to lower iPTH concentrations in volunteers with initially low 25OHD status, suggesting to utilize this simple form of treatment to improve vitamin D status and to have a possible biological effect on Ca homeostasis. However, we found no obvious effect on Sr absorption, suggesting the possibility that maximal vitamin D-dependent Ca absorption had already been achieved in these volunteers at a lower vitamin D status.
KW - 25-hydroxyvitamin D (25OHD)
KW - Calcium absorption
KW - Cholecalciferol (vitamin D)
KW - Optimal vitamin D levels
KW - Pakistan
KW - Strontium chloride (SrCl )
UR - http://www.scopus.com/inward/record.url?scp=84878845328&partnerID=8YFLogxK
U2 - 10.1007/s00198-012-2007-9
DO - 10.1007/s00198-012-2007-9
M3 - Article
C2 - 22572965
AN - SCOPUS:84878845328
SN - 0937-941X
VL - 24
SP - 1057
EP - 1062
JO - Osteoporosis International
JF - Osteoporosis International
IS - 3
ER -
