Non-invasive prenatal determination of fetal RhD genotyping from maternal plasma: A preliminary study in Pakistan

Nuruddin Mohammed, Fatima Kakal, Mehreen Somani, Wajiha Zafar

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


Objective: To determine the accuracy of the non-invasive pre-natal real-time polymerase chain reaction based fetal RhD genotyping from maternal plasma. Study Design: Cross-sectional study. Place and Duration of Study: Juma Health Sciences Research Laboratory, The Aga Khan University Hospital, Karachi, from July to December 2008. Methodology: Cell-free plasma DNA from 21 D-negative women with D-positive spouse between 20-39 weeks of gestation was tested for the presence of exon 5 region of RhD gene using real-time polymerase chain reaction, β-globin was employed as the house-keeping gene. Sensitivity and specificity of the real-time PCR-based non-invasive fetal RhD genotyping was obtained by calculating proportion of the D-positive fetuses that were D-positive at birth as well. Results: Of the 21 D-negative women 13 and 8 neonates were determined to be D-positive and D-negative, respectively, by serologic studies on cord blood samples at birth. RhD status was correctly determined in 17 of 21 cases. There were three false-positive and one false-negative results. The sensitivity and specificity of the assay was 92.3% (95% Cl: 62.1, 99.6) and 62.5% (95% Cl: 25.9,89.8), respectively. The positive and negative predictive value of the assay was 80% (95% Cl: 51.4, 94.7) and 83.3% (36.5, 99.1), respectively. Conclusion: These preliminary results demonstrate the feasibility of non-invasive pre-natal diagnosis of fetal RhD status of D-negative mothers in Pakistan.

Original languageEnglish
Pages (from-to)246-249
Number of pages4
JournalJournal of the College of Physicians and Surgeons--Pakistan : JCPSP
Issue number4
Publication statusPublished - Apr 2010


  • Cell-free fetal DNA
  • Non-invasive pre-natal diagnosis
  • Real-time polymerase chain reaction
  • RhD genotyping


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