Omicron spike function and neutralizing activity elicited by a comprehensive panel of vaccines

John E. Bowen; Amin Addetia; Ha V. Dang; Cameron Stewart; Jack T. Brown; William K. Sharkey; Kaitlin R. Sprouse; Alexandra C. Walls; Ignacio G. Mazzitelli; Jennifer K. Logue; Nicholas M. Franko; Nadine Czudnochowski; Abigail E. Powell; Exequiel Dellota; Kumail Ahmed; Asefa Shariq Ansari; Elisabetta Cameroni; Andrea Gori; Alessandra Bandera; Christine M. Posavad; Jennifer M. Dan; Zeli Zhang; Daniela Weiskopf; Alessandro Sette; Shane Crotty; Najeeha Talat Iqbal; Davide Corti; Jorge Geffner; Gyorgy Snell; Renata Grifantini; Helen Y. Chu; David Veesler

doi:10.1126/science.abq0203

Omicron spike function and neutralizing activity elicited by a comprehensive panel of vaccines

John E. Bowen
, Amin Addetia
, Ha V. Dang
, Cameron Stewart
, Jack T. Brown
, William K. Sharkey
, Kaitlin R. Sprouse
, Alexandra C. Walls
, Ignacio G. Mazzitelli
, Jennifer K. Logue
, Nicholas M. Franko
, Nadine Czudnochowski
, Abigail E. Powell
, Exequiel Dellota
, Kumail Ahmed
, Asefa Shariq Ansari
, Elisabetta Cameroni
, Andrea Gori
, Alessandra Bandera
, Christine M. Posavad

Jennifer M. Dan, Zeli Zhang, Daniela Weiskopf, Alessandro Sette, Shane Crotty, Najeeha Talat Iqbal, Davide Corti, Jorge Geffner, Gyorgy Snell, Renata Grifantini, Helen Y. Chu, David Veesler

Department of Biological & Biomedical Sciences, Medical College, Pakistan

Research output: Contribution to journal › Article › peer-review

131 Citations (Scopus)

Abstract

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron variant of concern comprises several sublineages, with BA.2 and BA.2.12.1 having replaced the previously dominant BA.1 and with BA.4 and BA.5 increasing in prevalence worldwide. We show that the large number of Omicron sublineage spike mutations leads to enhanced angiotensin-converting enzyme 2 (ACE2) binding, reduced fusogenicity, and severe dampening of plasma neutralizing activity elicited by infection or seven clinical vaccines relative to the ancestral virus. Administration of a homologous or heterologous booster based on the Wuhan-Hu-1 spike sequence markedly increased neutralizing antibody titers and breadth against BA.1, BA.2, BA.2.12.1, BA.4, and BA.5 across all vaccines evaluated. Our data suggest that although Omicron sublineages evade polyclonal neutralizing antibody responses elicited by primary vaccine series, vaccine boosters may provide sufficient protection against Omicron-induced severe disease.

Original language	English (US)
Pages (from-to)	890-894
Number of pages	5
Journal	Science
Volume	377
Issue number	6608
DOIs	https://doi.org/10.1126/science.abq0203
Publication status	Published - 19 Aug 2022

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SDG 3 Good Health and Well-being

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10.1126/science.abq0203

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Bowen, J. E., Addetia, A., Dang, H. V., Stewart, C., Brown, J. T., Sharkey, W. K., Sprouse, K. R., Walls, A. C., Mazzitelli, I. G., Logue, J. K., Franko, N. M., Czudnochowski, N., Powell, A. E., Dellota, E., Ahmed, K., Ansari, A. S., Cameroni, E., Gori, A., Bandera, A., ... Veesler, D. (2022). Omicron spike function and neutralizing activity elicited by a comprehensive panel of vaccines. Science, 377(6608), 890-894. https://doi.org/10.1126/science.abq0203

@article{47efae36e5214e149905d90720532a04,

title = "Omicron spike function and neutralizing activity elicited by a comprehensive panel of vaccines",

abstract = "The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron variant of concern comprises several sublineages, with BA.2 and BA.2.12.1 having replaced the previously dominant BA.1 and with BA.4 and BA.5 increasing in prevalence worldwide. We show that the large number of Omicron sublineage spike mutations leads to enhanced angiotensin-converting enzyme 2 (ACE2) binding, reduced fusogenicity, and severe dampening of plasma neutralizing activity elicited by infection or seven clinical vaccines relative to the ancestral virus. Administration of a homologous or heterologous booster based on the Wuhan-Hu-1 spike sequence markedly increased neutralizing antibody titers and breadth against BA.1, BA.2, BA.2.12.1, BA.4, and BA.5 across all vaccines evaluated. Our data suggest that although Omicron sublineages evade polyclonal neutralizing antibody responses elicited by primary vaccine series, vaccine boosters may provide sufficient protection against Omicron-induced severe disease.",

author = "Bowen, \{John E.\} and Amin Addetia and Dang, \{Ha V.\} and Cameron Stewart and Brown, \{Jack T.\} and Sharkey, \{William K.\} and Sprouse, \{Kaitlin R.\} and Walls, \{Alexandra C.\} and Mazzitelli, \{Ignacio G.\} and Logue, \{Jennifer K.\} and Franko, \{Nicholas M.\} and Nadine Czudnochowski and Powell, \{Abigail E.\} and Exequiel Dellota and Kumail Ahmed and Ansari, \{Asefa Shariq\} and Elisabetta Cameroni and Andrea Gori and Alessandra Bandera and Posavad, \{Christine M.\} and Dan, \{Jennifer M.\} and Zeli Zhang and Daniela Weiskopf and Alessandro Sette and Shane Crotty and Iqbal, \{Najeeha Talat\} and Davide Corti and Jorge Geffner and Gyorgy Snell and Renata Grifantini and Chu, \{Helen Y.\} and David Veesler",

year = "2022",

month = aug,

day = "19",

doi = "10.1126/science.abq0203",

language = "English (US)",

volume = "377",

pages = "890--894",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "6608",

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Bowen, JE, Addetia, A, Dang, HV, Stewart, C, Brown, JT, Sharkey, WK, Sprouse, KR, Walls, AC, Mazzitelli, IG, Logue, JK, Franko, NM, Czudnochowski, N, Powell, AE, Dellota, E, Ahmed, K, Ansari, AS, Cameroni, E, Gori, A, Bandera, A, Posavad, CM, Dan, JM, Zhang, Z, Weiskopf, D, Sette, A, Crotty, S, Iqbal, NT, Corti, D, Geffner, J, Snell, G, Grifantini, R, Chu, HY & Veesler, D 2022, 'Omicron spike function and neutralizing activity elicited by a comprehensive panel of vaccines', Science, vol. 377, no. 6608, pp. 890-894. https://doi.org/10.1126/science.abq0203

TY - JOUR

T1 - Omicron spike function and neutralizing activity elicited by a comprehensive panel of vaccines

AU - Bowen, John E.

AU - Addetia, Amin

AU - Dang, Ha V.

AU - Stewart, Cameron

AU - Brown, Jack T.

AU - Sharkey, William K.

AU - Sprouse, Kaitlin R.

AU - Walls, Alexandra C.

AU - Mazzitelli, Ignacio G.

AU - Logue, Jennifer K.

AU - Franko, Nicholas M.

AU - Czudnochowski, Nadine

AU - Powell, Abigail E.

AU - Dellota, Exequiel

AU - Ahmed, Kumail

AU - Ansari, Asefa Shariq

AU - Cameroni, Elisabetta

AU - Gori, Andrea

AU - Bandera, Alessandra

AU - Posavad, Christine M.

AU - Dan, Jennifer M.

AU - Zhang, Zeli

AU - Weiskopf, Daniela

AU - Sette, Alessandro

AU - Crotty, Shane

AU - Iqbal, Najeeha Talat

AU - Corti, Davide

AU - Geffner, Jorge

AU - Snell, Gyorgy

AU - Grifantini, Renata

AU - Chu, Helen Y.

AU - Veesler, David

PY - 2022/8/19

Y1 - 2022/8/19

N2 - The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron variant of concern comprises several sublineages, with BA.2 and BA.2.12.1 having replaced the previously dominant BA.1 and with BA.4 and BA.5 increasing in prevalence worldwide. We show that the large number of Omicron sublineage spike mutations leads to enhanced angiotensin-converting enzyme 2 (ACE2) binding, reduced fusogenicity, and severe dampening of plasma neutralizing activity elicited by infection or seven clinical vaccines relative to the ancestral virus. Administration of a homologous or heterologous booster based on the Wuhan-Hu-1 spike sequence markedly increased neutralizing antibody titers and breadth against BA.1, BA.2, BA.2.12.1, BA.4, and BA.5 across all vaccines evaluated. Our data suggest that although Omicron sublineages evade polyclonal neutralizing antibody responses elicited by primary vaccine series, vaccine boosters may provide sufficient protection against Omicron-induced severe disease.

AB - The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron variant of concern comprises several sublineages, with BA.2 and BA.2.12.1 having replaced the previously dominant BA.1 and with BA.4 and BA.5 increasing in prevalence worldwide. We show that the large number of Omicron sublineage spike mutations leads to enhanced angiotensin-converting enzyme 2 (ACE2) binding, reduced fusogenicity, and severe dampening of plasma neutralizing activity elicited by infection or seven clinical vaccines relative to the ancestral virus. Administration of a homologous or heterologous booster based on the Wuhan-Hu-1 spike sequence markedly increased neutralizing antibody titers and breadth against BA.1, BA.2, BA.2.12.1, BA.4, and BA.5 across all vaccines evaluated. Our data suggest that although Omicron sublineages evade polyclonal neutralizing antibody responses elicited by primary vaccine series, vaccine boosters may provide sufficient protection against Omicron-induced severe disease.

UR - https://www.scopus.com/pages/publications/85136220432

U2 - 10.1126/science.abq0203

DO - 10.1126/science.abq0203

M3 - Article

C2 - 35857529

AN - SCOPUS:85136220432

SN - 0036-8075

VL - 377

SP - 890

EP - 894

JO - Science

JF - Science

IS - 6608

ER -

Omicron spike function and neutralizing activity elicited by a comprehensive panel of vaccines

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