Optical map of the genotype A1 WB C6 Giardia lamblia genome isolate

D. Alexander Perry; Hilary G. Morrison; Rodney D. Adam

doi:10.1016/j.molbiopara.2011.07.008

Optical map of the genotype A1 WB C6 Giardia lamblia genome isolate

D. Alexander Perry, Hilary G. Morrison, Rodney D. Adam

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

The Giardia lamblia genome consists of 12 Mb divided among 5 chromosomes ranging in size from approximately 1 to 4 Mb. The assembled contigs of the genotype A1 isolate, WB, were previously mapped along the 5 chromosomes on the basis of hybridization of plasmid clones representing the contigs to chromosomes separated by PFGE. In the current report, we have generated an MluI optical map of the WB genome to improve the accuracy of the physical map. This has allowed us to correct several assembly errors and to better define the extent of the subtelomeric regions that are not included in the genome assembly.

Original language	English
Pages (from-to)	112-114
Number of pages	3
Journal	Molecular and Biochemical Parasitology
Volume	180
Issue number	2
DOIs	https://doi.org/10.1016/j.molbiopara.2011.07.008
Publication status	Published - Dec 2011
Externally published	Yes

Keywords

Genome
Optical map
Pulsed field gel electrophoresis
Subtelomeric variation

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10.1016/j.molbiopara.2011.07.008

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title = "Optical map of the genotype A1 WB C6 Giardia lamblia genome isolate",

abstract = "The Giardia lamblia genome consists of 12 Mb divided among 5 chromosomes ranging in size from approximately 1 to 4 Mb. The assembled contigs of the genotype A1 isolate, WB, were previously mapped along the 5 chromosomes on the basis of hybridization of plasmid clones representing the contigs to chromosomes separated by PFGE. In the current report, we have generated an MluI optical map of the WB genome to improve the accuracy of the physical map. This has allowed us to correct several assembly errors and to better define the extent of the subtelomeric regions that are not included in the genome assembly.",

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author = "Perry, {D. Alexander} and Morrison, {Hilary G.} and Adam, {Rodney D.}",

note = "Funding Information: This work was funded in part by the Woods Hole Center for Oceans and Human Health , jointly funded by the National Science Foundation (OCE- 0430724 ) and the National Institute for Environmental Health Sciences (P50 ES012742 ). ",

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AU - Morrison, Hilary G.

AU - Adam, Rodney D.

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KW - Pulsed field gel electrophoresis

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Optical map of the genotype A1 WB C6 Giardia lamblia genome isolate

Abstract

Keywords

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