Performance evaluation of ion exchange and affinity chromatography for HbA1c estimation in diabetic patients with HbD: A study of 129 samples

Bushra Moiz; Mashhooda Rasool Hashmi; Shahmina Sadaf

doi:10.1016/j.clinbiochem.2008.06.015

Performance evaluation of ion exchange and affinity chromatography for HbA_1c estimation in diabetic patients with HbD: A study of 129 samples

Bushra Moiz, Mashhooda Rasool Hashmi, Shahmina Sadaf

Department of Pathology and Laboratory Medicine, Medical College, Pakistan

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Objectives: To compare ion exchange and boronate affinity chromatography for HbA_1c estimation in patients with type I and II diabetes having hemoglobin D. Design and methods: Systems based on ion exchange and boronate affinity chromatography were evaluated and compared for their performance for HbA_1c estimation in patients with homozygous and heterozygous D disease. Results: Boronate affinity chromatography shows least interference by HbD in heterozygous as well as homozygous diabetic patients for HbA_1c estimation. Conclusions: The use of boronate affinity chromatography was found to be helpful in evaluating glycemic control in diabetic subjects with HbD.

Original language	English
Pages (from-to)	1204-1210
Number of pages	7
Journal	Clinical Biochemistry
Volume	41
Issue number	14-15
DOIs	https://doi.org/10.1016/j.clinbiochem.2008.06.015
Publication status	Published - Oct 2008

Keywords

Chromatography
Diabetes
HbA
HbD
Hemoglobin variant

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.clinbiochem.2008.06.015

Cite this

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title = "Performance evaluation of ion exchange and affinity chromatography for HbA1c estimation in diabetic patients with HbD: A study of 129 samples",

abstract = "Objectives: To compare ion exchange and boronate affinity chromatography for HbA1c estimation in patients with type I and II diabetes having hemoglobin D. Design and methods: Systems based on ion exchange and boronate affinity chromatography were evaluated and compared for their performance for HbA1c estimation in patients with homozygous and heterozygous D disease. Results: Boronate affinity chromatography shows least interference by HbD in heterozygous as well as homozygous diabetic patients for HbA1c estimation. Conclusions: The use of boronate affinity chromatography was found to be helpful in evaluating glycemic control in diabetic subjects with HbD.",

keywords = "Chromatography, Diabetes, HbA, HbD, Hemoglobin variant",

author = "Bushra Moiz and {Rasool Hashmi}, Mashhooda and Shahmina Sadaf",

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language = "English",

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T2 - A study of 129 samples

AU - Moiz, Bushra

AU - Rasool Hashmi, Mashhooda

AU - Sadaf, Shahmina

PY - 2008/10

Y1 - 2008/10

N2 - Objectives: To compare ion exchange and boronate affinity chromatography for HbA1c estimation in patients with type I and II diabetes having hemoglobin D. Design and methods: Systems based on ion exchange and boronate affinity chromatography were evaluated and compared for their performance for HbA1c estimation in patients with homozygous and heterozygous D disease. Results: Boronate affinity chromatography shows least interference by HbD in heterozygous as well as homozygous diabetic patients for HbA1c estimation. Conclusions: The use of boronate affinity chromatography was found to be helpful in evaluating glycemic control in diabetic subjects with HbD.

AB - Objectives: To compare ion exchange and boronate affinity chromatography for HbA1c estimation in patients with type I and II diabetes having hemoglobin D. Design and methods: Systems based on ion exchange and boronate affinity chromatography were evaluated and compared for their performance for HbA1c estimation in patients with homozygous and heterozygous D disease. Results: Boronate affinity chromatography shows least interference by HbD in heterozygous as well as homozygous diabetic patients for HbA1c estimation. Conclusions: The use of boronate affinity chromatography was found to be helpful in evaluating glycemic control in diabetic subjects with HbD.

KW - Chromatography

KW - Diabetes

KW - HbA

KW - HbD

KW - Hemoglobin variant

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