Pharmacological studies on hypotensive, diuretic and vasodilator activities of chrysin glucoside from Calycotome villosa in rats

K. Cherkaoui-Tangi, M. Lachkar, M. Wibo, N. Morel, Anwarul Hassan Gilani, B. Lyoussi

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20 Citations (Scopus)


The present study was undertaken in normotensive anaesthetized male rats that received a continuous perfusion of a chrysin glucoside isolated from the flowers and leaves of Calycotome villosa subsp intermedia at a dose of 2.5 mg/kg, or furosemide (control diuretic) at a dose of 0.5 mg/kg. Compared with the control rats receiving NaCl (0.9%), the urine flow, glomerular filtration and electrolyte excretion (Na+, K+) increased significantly in rats treated with chrysin glucoside (p < 0.001). A similar effect was observed in the rats perfused with furosemide. Intravenous injections of bolus doses (1-3 mg/kg) of the chrysin glucoside to anaesthetized rats elicited an immediate and dose-dependent decrease in mean arterial blood pressure (MABP). Pretreatment of the rats with the nitric oxide synthase inhibitor, L-NOArg (10 mg/kg), reduced partially, but significantly (p < 0.01), the maximal decrease in MABP elicited by chrysin glucoside. In the rat isolated aorta preparation, chrysin glucoside (10-100 μM) inhibited in a concentration-dependent manner the noradrenaline (1 μM) induced contractions (IC50 = 52 μM). This relaxant activity of chrysin glucoside was significantly reduced by incubation of the endothelium-intact rings with L-NOArg (100 μM), (80 ± 4.7% vs 48 ± 5.06% in the absence of L-NOArg). In conclusion, these results demonstrate a diuretic and hypotensive action of a chrysin glucoside from Calycotome villosa in anaesthetized rats and indicating an action on renal function, and an active vascular relaxation mediated partially through nitric oxide release.

Original languageEnglish
Pages (from-to)356-361
Number of pages6
JournalPhytotherapy Research
Issue number3
Publication statusPublished - Mar 2008
Externally publishedYes


  • Calycotome villosa
  • Chrysin glucoside
  • Diuretic
  • Endothelial dependent
  • Hypotensive
  • Rats
  • Vasorelaxant


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