Plasmid mediated antibacterial resistance in Salmonella typhi

B. Farooqi; F. Shareeq; Q. K. Rizvi; M. Khurshid; N. Ahmed; N. Siddiqa; H. S. Qureshi; M. S. Mahmood; M. K. Ashfaq

Plasmid mediated antibacterial resistance in Salmonella typhi

B. Farooqi, F. Shareeq, Q. K. Rizvi, M. Khurshid, N. Ahmed, N. Siddiqa, H. S. Qureshi, M. S. Mahmood, M. K. Ashfaq

Section of Haematology/Oncology, Department of Medicine, Medical College, Pakistan

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Salmonella typhi resistant to chloramphenicol, ampicillin and trimethoprim-sulphamethoxazole are simultaneously increasing in number worldwide. We isolated 290 Multiple drug resistant (MDR) S. typhi with high range of Minimum Inhibitory Concentrations (MICs). These isolates were sensitive to cefotaxime, ceftriaxone, zetreonam and ofloxacin with a narrow range of MICs. Tansconjugants E. coli showed same antibiogram and MICs as donor cells. All MDR S. typhi isolates and transconjugants contained a single plasmid pBN1 of 20kb. Purified plasmid of MDR S. typhi and transconjugants when treated with restriction endonuclease enzyme EcoR1 revealed no restriction site for EcoR1.

Original language	English
Pages (from-to)	107-109
Number of pages	3
Journal	Journal of the College of Physicians and Surgeons--Pakistan : JCPSP
Volume	11
Issue number	2
Publication status	Published - 2001

Keywords

Antibiotics
Drug resistance, multiple
Microbial sensitivity tests
Plasmids
Salmonella typhi

Cite this

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title = "Plasmid mediated antibacterial resistance in Salmonella typhi",

abstract = "Salmonella typhi resistant to chloramphenicol, ampicillin and trimethoprim-sulphamethoxazole are simultaneously increasing in number worldwide. We isolated 290 Multiple drug resistant (MDR) S. typhi with high range of Minimum Inhibitory Concentrations (MICs). These isolates were sensitive to cefotaxime, ceftriaxone, zetreonam and ofloxacin with a narrow range of MICs. Tansconjugants E. coli showed same antibiogram and MICs as donor cells. All MDR S. typhi isolates and transconjugants contained a single plasmid pBN1 of 20kb. Purified plasmid of MDR S. typhi and transconjugants when treated with restriction endonuclease enzyme EcoR1 revealed no restriction site for EcoR1.",

keywords = "Antibiotics, Drug resistance, multiple, Microbial sensitivity tests, Plasmids, Salmonella typhi",

author = "B. Farooqi and F. Shareeq and Rizvi, {Q. K.} and M. Khurshid and N. Ahmed and N. Siddiqa and Qureshi, {H. S.} and Mahmood, {M. S.} and Ashfaq, {M. K.}",

year = "2001",

language = "English",

volume = "11",

pages = "107--109",

journal = "Journal of the College of Physicians and Surgeons--Pakistan : JCPSP",

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TY - JOUR

T1 - Plasmid mediated antibacterial resistance in Salmonella typhi

AU - Farooqi, B.

AU - Shareeq, F.

AU - Rizvi, Q. K.

AU - Khurshid, M.

AU - Ahmed, N.

AU - Siddiqa, N.

AU - Qureshi, H. S.

AU - Mahmood, M. S.

AU - Ashfaq, M. K.

PY - 2001

Y1 - 2001

N2 - Salmonella typhi resistant to chloramphenicol, ampicillin and trimethoprim-sulphamethoxazole are simultaneously increasing in number worldwide. We isolated 290 Multiple drug resistant (MDR) S. typhi with high range of Minimum Inhibitory Concentrations (MICs). These isolates were sensitive to cefotaxime, ceftriaxone, zetreonam and ofloxacin with a narrow range of MICs. Tansconjugants E. coli showed same antibiogram and MICs as donor cells. All MDR S. typhi isolates and transconjugants contained a single plasmid pBN1 of 20kb. Purified plasmid of MDR S. typhi and transconjugants when treated with restriction endonuclease enzyme EcoR1 revealed no restriction site for EcoR1.

AB - Salmonella typhi resistant to chloramphenicol, ampicillin and trimethoprim-sulphamethoxazole are simultaneously increasing in number worldwide. We isolated 290 Multiple drug resistant (MDR) S. typhi with high range of Minimum Inhibitory Concentrations (MICs). These isolates were sensitive to cefotaxime, ceftriaxone, zetreonam and ofloxacin with a narrow range of MICs. Tansconjugants E. coli showed same antibiogram and MICs as donor cells. All MDR S. typhi isolates and transconjugants contained a single plasmid pBN1 of 20kb. Purified plasmid of MDR S. typhi and transconjugants when treated with restriction endonuclease enzyme EcoR1 revealed no restriction site for EcoR1.

KW - Antibiotics

KW - Drug resistance, multiple

KW - Microbial sensitivity tests

KW - Plasmids

KW - Salmonella typhi

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M3 - Article

AN - SCOPUS:0035032697

SN - 1022-386X

VL - 11

SP - 107

EP - 109

JO - Journal of the College of Physicians and Surgeons--Pakistan : JCPSP

JF - Journal of the College of Physicians and Surgeons--Pakistan : JCPSP

IS - 2

ER -

Plasmid mediated antibacterial resistance in Salmonella typhi

Abstract

Keywords

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