Population diversity of sheep bot fly, Oestrus ovis (Linné, 1758) (Diptera: Oestridae: Oestrinae), using SDS polyacrylamide gel electrophoresis

Mustafa Kamal, Ghazala Yasmeen, Noor Us Saher, Farah Naz, Naseem Rafiq, Wali Khan, Muhammad Anwar Iqbal, Muhammad Kabir, Safia Mushtaq, Habib Ul Hassan, Tahir Usman

Research output: Contribution to journalArticlepeer-review

Abstract

The study was planned to evaluate the inter, and intra population genetic variation in general protein banding pattern in Oestrus ovis larvae, by using 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE). The larvae were collected from slaughtered goats head from five different locations (AAS, PN, LA, GM, and BC) of Karachi, Pakistan. The data obtained was subjected to POPGENE (Population Genetic Analysis) software for analysis. The polymorphic loci within populations ranged from 45.45% to 90.91%. Polymorphic loci observed in all populations were 90.91%. The expected heterozygosity observed was 0.182 ± 0.096 in all populations. The chi-square test showed 5 out of 11 loci at H-W equilibrium. The overall fixation index (FST) value was 0.108, showing that the likelihood of subpopulations being differentiated from one another is about 11 percent. The gene flow value (Nm = 2.065) was higher, showing that genes flow occurs between populations. The values of genetic identity were greater, and genetic distance were smaller among all the populations, which means that all the populations were more alike and closer to each other. It was concluded that there was no sympatric and parapatric population differentiation observed among all the population of O. ovis and the populations of the five different locations were not genetically and reproductively isolated from each other.

Original languageEnglish
Pages (from-to)1694-1698
Number of pages5
JournalSaudi Journal of Biological Sciences
Volume29
Issue number3
DOIs
Publication statusPublished - Mar 2022
Externally publishedYes

Keywords

  • Karachi
  • Oestrus ovis
  • Population diversity
  • Protein
  • SDS PAGE

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