Presence of blood-pressure lowering and spasmolytic constituents in buddleja crispa

Anwarul H. Gilani, Ishfaq A. Bukhari, Rafeeq A. Khan, Abdul J. Shah, Ijaz Ahmad, Abdul Malik

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7 Citations (Scopus)


This aim of this study was to investigate the crude extract of Buddleja crispa (Bc.Cr) and its active constituent(s) for their antihypertensive and antispasmodic activities. The Bc.Cr caused a dose-dependent (3-10 mg/kg) fall in mean arterial pressure in rats under anesthesia. In rabbit aorta preparations, Bc.Cr (0.03-1 mg/mL) caused inhibition of high K+ (80 mM) precontractions. The Bc.Cr (0.03-1 mg/mL) also inhibited spontaneous and high K+ precontractions in rabbit jejunum preparations, suggestive of calcium channel blocking (CCB) activity. CCB activity was further confirmed when pretreatment of the tissues with Bc.Cr (0.03-0.10 mg/mL) caused a rightward shift in Ca " concentration response curves, similar to verapamil. Among the pure compounds, BdI-H3 was more potent against the high K+ than spontaneous contractions and was around eight times more potent than Bc.Cr against the spontaneous contractions while the other two compounds, BdI-2 and BH-3 were inactive. Activity-directed fractionation revealed that the hexane fraction was more potent against K+ precontractions. These data indicate that Bc.Cr possesses a blood-pressure lowering effect, mediated possibly through CCB, though additional mechanism(s) cannot be ruled out. Among the pure compounds, Bdl-H3 is likely to be the active compound involved in the spasmolytic and possibly BP lowering effect of the parent crude extract.

Original languageEnglish
Pages (from-to)492-497
Number of pages6
JournalPhytotherapy Research
Issue number4
Publication statusPublished - Apr 2009
Externally publishedYes


  • Buddleja crispa
  • Calcium antagonist
  • Fractionation
  • Hypotensive
  • Pure compounds
  • Spasmolytic


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