Prevalence and molecular diagnosis of Trypanosoma evansi in Nili-Ravi buffalo (Bubalus bubalis) in different districts of Punjab (Pakistan)

Waseem Shahzad; Rashid Munir; Mohammad S. Khan; Mansur D. Ahmad; Mohammad Ijaz; Ashfaq Ahmad; Mohammad Iqbal

doi:10.1007/s11250-010-9616-0

Prevalence and molecular diagnosis of Trypanosoma evansi in Nili-Ravi buffalo (Bubalus bubalis) in different districts of Punjab (Pakistan)

Waseem Shahzad, Rashid Munir, Mohammad S. Khan, Mansur D. Ahmad, Mohammad Ijaz, Ashfaq Ahmad, Mohammad Iqbal

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

The prevalence of Trypanosoma evansi was investigated in 1,250 Nili-Ravi buffaloes of mixed age and sex by polymerase chain reaction (PCR) for the first time in Pakistan. DNA of the trypanosomes was isolated with TRIREAGENT®. The assay was employed using primers ESAG 6/7, specific for a 237-bp fragment from T. evansi genomic DNA. The samples were screened for the presence of T. evansi also by stained thin smear. Forty-four (3. 5%) samples were positive by microscopy, while 97 (7. 7%) samples were identified by PCR, indicating the high sensitivity of PCR for surveying the disease in epidemiological studies.

Original language	English
Pages (from-to)	1597-1599
Number of pages	3
Journal	Tropical Animal Health and Production
Volume	42
Issue number	8
DOIs	https://doi.org/10.1007/s11250-010-9616-0
Publication status	Published - Dec 2010
Externally published	Yes

Keywords

Bovine
Polymerase chain reaction
T. evansi
Trypanosomiasis

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10.1007/s11250-010-9616-0

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title = "Prevalence and molecular diagnosis of Trypanosoma evansi in Nili-Ravi buffalo (Bubalus bubalis) in different districts of Punjab (Pakistan)",

abstract = "The prevalence of Trypanosoma evansi was investigated in 1,250 Nili-Ravi buffaloes of mixed age and sex by polymerase chain reaction (PCR) for the first time in Pakistan. DNA of the trypanosomes was isolated with TRIREAGENT{\textregistered}. The assay was employed using primers ESAG 6/7, specific for a 237-bp fragment from T. evansi genomic DNA. The samples were screened for the presence of T. evansi also by stained thin smear. Forty-four (3. 5%) samples were positive by microscopy, while 97 (7. 7%) samples were identified by PCR, indicating the high sensitivity of PCR for surveying the disease in epidemiological studies.",

keywords = "Bovine, Polymerase chain reaction, T. evansi, Trypanosomiasis",

author = "Waseem Shahzad and Rashid Munir and Khan, {Mohammad S.} and Ahmad, {Mansur D.} and Mohammad Ijaz and Ashfaq Ahmad and Mohammad Iqbal",

year = "2010",

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doi = "10.1007/s11250-010-9616-0",

language = "English",

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AU - Shahzad, Waseem

AU - Munir, Rashid

AU - Khan, Mohammad S.

AU - Ahmad, Mansur D.

AU - Ijaz, Mohammad

AU - Ahmad, Ashfaq

AU - Iqbal, Mohammad

PY - 2010/12

Y1 - 2010/12

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AB - The prevalence of Trypanosoma evansi was investigated in 1,250 Nili-Ravi buffaloes of mixed age and sex by polymerase chain reaction (PCR) for the first time in Pakistan. DNA of the trypanosomes was isolated with TRIREAGENT®. The assay was employed using primers ESAG 6/7, specific for a 237-bp fragment from T. evansi genomic DNA. The samples were screened for the presence of T. evansi also by stained thin smear. Forty-four (3. 5%) samples were positive by microscopy, while 97 (7. 7%) samples were identified by PCR, indicating the high sensitivity of PCR for surveying the disease in epidemiological studies.

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KW - Polymerase chain reaction

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KW - Trypanosomiasis

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Prevalence and molecular diagnosis of Trypanosoma evansi in Nili-Ravi buffalo (Bubalus bubalis) in different districts of Punjab (Pakistan)

Abstract

Keywords

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