Prevalence and molecular diagnosis of Trypanosoma evansi in Nili-Ravi buffalo (Bubalus bubalis) in different districts of Punjab (Pakistan)

Waseem Shahzad, Rashid Munir, Mohammad S. Khan, Mansur D. Ahmad, Mohammad Ijaz, Ashfaq Ahmad, Mohammad Iqbal

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

The prevalence of Trypanosoma evansi was investigated in 1,250 Nili-Ravi buffaloes of mixed age and sex by polymerase chain reaction (PCR) for the first time in Pakistan. DNA of the trypanosomes was isolated with TRIREAGENT®. The assay was employed using primers ESAG 6/7, specific for a 237-bp fragment from T. evansi genomic DNA. The samples were screened for the presence of T. evansi also by stained thin smear. Forty-four (3. 5%) samples were positive by microscopy, while 97 (7. 7%) samples were identified by PCR, indicating the high sensitivity of PCR for surveying the disease in epidemiological studies.

Original languageEnglish
Pages (from-to)1597-1599
Number of pages3
JournalTropical Animal Health and Production
Volume42
Issue number8
DOIs
Publication statusPublished - Dec 2010
Externally publishedYes

Keywords

  • Bovine
  • Polymerase chain reaction
  • T. evansi
  • Trypanosomiasis

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