Prevalence and molecular diagnosis of Trypanosoma evansi in Nili-Ravi buffalo (Bubalus bubalis) in different districts of Punjab (Pakistan)

Waseem Shahzad; Rashid Munir; Mohammad S. Khan; Mansur D. Ahmad; Mohammad Ijaz; Ashfaq Ahmad; Mohammad Iqbal

doi:10.1007/s11250-010-9616-0

Prevalence and molecular diagnosis of Trypanosoma evansi in Nili-Ravi buffalo (Bubalus bubalis) in different districts of Punjab (Pakistan)

Waseem Shahzad, Rashid Munir, Mohammad S. Khan, Mansur D. Ahmad, Mohammad Ijaz, Ashfaq Ahmad, Mohammad Iqbal

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

The prevalence of Trypanosoma evansi was investigated in 1,250 Nili-Ravi buffaloes of mixed age and sex by polymerase chain reaction (PCR) for the first time in Pakistan. DNA of the trypanosomes was isolated with TRIREAGENT®. The assay was employed using primers ESAG 6/7, specific for a 237-bp fragment from T. evansi genomic DNA. The samples were screened for the presence of T. evansi also by stained thin smear. Forty-four (3. 5%) samples were positive by microscopy, while 97 (7. 7%) samples were identified by PCR, indicating the high sensitivity of PCR for surveying the disease in epidemiological studies.

Original language	English (UK)
Pages (from-to)	1597-1599
Number of pages	3
Journal	Tropical Animal Health and Production
Volume	42
Issue number	8
DOIs	https://doi.org/10.1007/s11250-010-9616-0
Publication status	Published - Dec 2010
Externally published	Yes

Keywords

Bovine
Polymerase chain reaction
T. evansi
Trypanosomiasis

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10.1007/s11250-010-9616-0

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title = "Prevalence and molecular diagnosis of Trypanosoma evansi in Nili-Ravi buffalo (Bubalus bubalis) in different districts of Punjab (Pakistan)",

abstract = "The prevalence of Trypanosoma evansi was investigated in 1,250 Nili-Ravi buffaloes of mixed age and sex by polymerase chain reaction (PCR) for the first time in Pakistan. DNA of the trypanosomes was isolated with TRIREAGENT{\textregistered}. The assay was employed using primers ESAG 6/7, specific for a 237-bp fragment from T. evansi genomic DNA. The samples were screened for the presence of T. evansi also by stained thin smear. Forty-four (3. 5\%) samples were positive by microscopy, while 97 (7. 7\%) samples were identified by PCR, indicating the high sensitivity of PCR for surveying the disease in epidemiological studies.",

keywords = "Bovine, Polymerase chain reaction, T. evansi, Trypanosomiasis",

author = "Waseem Shahzad and Rashid Munir and Khan, \{Mohammad S.\} and Ahmad, \{Mansur D.\} and Mohammad Ijaz and Ashfaq Ahmad and Mohammad Iqbal",

year = "2010",

month = dec,

doi = "10.1007/s11250-010-9616-0",

language = "English (UK)",

volume = "42",

pages = "1597--1599",

journal = "Tropical Animal Health and Production",

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T1 - Prevalence and molecular diagnosis of Trypanosoma evansi in Nili-Ravi buffalo (Bubalus bubalis) in different districts of Punjab (Pakistan)

AU - Shahzad, Waseem

AU - Munir, Rashid

AU - Khan, Mohammad S.

AU - Ahmad, Mansur D.

AU - Ijaz, Mohammad

AU - Ahmad, Ashfaq

AU - Iqbal, Mohammad

PY - 2010/12

Y1 - 2010/12

N2 - The prevalence of Trypanosoma evansi was investigated in 1,250 Nili-Ravi buffaloes of mixed age and sex by polymerase chain reaction (PCR) for the first time in Pakistan. DNA of the trypanosomes was isolated with TRIREAGENT®. The assay was employed using primers ESAG 6/7, specific for a 237-bp fragment from T. evansi genomic DNA. The samples were screened for the presence of T. evansi also by stained thin smear. Forty-four (3. 5%) samples were positive by microscopy, while 97 (7. 7%) samples were identified by PCR, indicating the high sensitivity of PCR for surveying the disease in epidemiological studies.

AB - The prevalence of Trypanosoma evansi was investigated in 1,250 Nili-Ravi buffaloes of mixed age and sex by polymerase chain reaction (PCR) for the first time in Pakistan. DNA of the trypanosomes was isolated with TRIREAGENT®. The assay was employed using primers ESAG 6/7, specific for a 237-bp fragment from T. evansi genomic DNA. The samples were screened for the presence of T. evansi also by stained thin smear. Forty-four (3. 5%) samples were positive by microscopy, while 97 (7. 7%) samples were identified by PCR, indicating the high sensitivity of PCR for surveying the disease in epidemiological studies.

KW - Bovine

KW - Polymerase chain reaction

KW - T. evansi

KW - Trypanosomiasis

UR - https://www.scopus.com/pages/publications/78049396084

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DO - 10.1007/s11250-010-9616-0

M3 - Article

C2 - 20680446

AN - SCOPUS:78049396084

SN - 0049-4747

VL - 42

SP - 1597

EP - 1599

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ER -

Prevalence and molecular diagnosis of Trypanosoma evansi in Nili-Ravi buffalo (Bubalus bubalis) in different districts of Punjab (Pakistan)

Abstract

Keywords

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