Prevalence of carbapenemase-producing organisms at the Kidney Center of Rawalpindi (Pakistan) and evaluation of an advanced molecular microarray-based carbapenemase assay

Sascha D. Braun; Bushra Jamil; Muhammad A. Syed; Shahid A. Abbasi; Daniel Weiß; Peter Slickers; Stefan Monecke; Ines Engelmann; Ralf Ehricht

doi:10.2217/fmb-2018-0082

Prevalence of carbapenemase-producing organisms at the Kidney Center of Rawalpindi (Pakistan) and evaluation of an advanced molecular microarray-based carbapenemase assay

Sascha D. Braun
, Bushra Jamil
, Muhammad A. Syed
, Shahid A. Abbasi
, Daniel Weiß
, Peter Slickers
, Stefan Monecke
, Ines Engelmann
, Ralf Ehricht

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

Aim: A DNA microarray-based assay for the detection of antimicrobial resistance (AMR) genes was used to study carbapenemase-producing organisms at the Kidney Center of Rawalpindi, Pakistan. Methods: The evaluation of this assay was performed using 97 reference strains with confirmed AMR genes. Testing of 7857 clinical samples identified 425 Gram-negative bacteria out of which 82 appeared carbapenem resistant. These isolates were analyzed using VITEK-2 for phenotyping and the described AMR assay for genotyping. Results: The most prevalent carbapenemase gene was blaNDM and in 12 isolates we detected two carbapenemase genes (e.g., blaNDM/blaOXA-48). Conclusion: Our prevalence data from Pakistan show that-as in other parts of the world-carbapenemase-producing organisms with different underlying resistance mechanisms are emerging, and this warrants intensified and constant surveillance.

Original language	English (UK)
Pages (from-to)	1225-1246
Number of pages	22
Journal	Future Microbiology
Volume	13
Issue number	11
DOIs	https://doi.org/10.2217/fmb-2018-0082
Publication status	Published - Sept 2018
Externally published	Yes

Keywords

Pakistan

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10.2217/fmb-2018-0082

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Braun, S. D., Jamil, B., Syed, M. A., Abbasi, S. A., Weiß, D., Slickers, P., Monecke, S., Engelmann, I., & Ehricht, R. (2018). Prevalence of carbapenemase-producing organisms at the Kidney Center of Rawalpindi (Pakistan) and evaluation of an advanced molecular microarray-based carbapenemase assay. Future Microbiology, 13(11), 1225-1246. https://doi.org/10.2217/fmb-2018-0082

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title = "Prevalence of carbapenemase-producing organisms at the Kidney Center of Rawalpindi (Pakistan) and evaluation of an advanced molecular microarray-based carbapenemase assay",

abstract = "Aim: A DNA microarray-based assay for the detection of antimicrobial resistance (AMR) genes was used to study carbapenemase-producing organisms at the Kidney Center of Rawalpindi, Pakistan. Methods: The evaluation of this assay was performed using 97 reference strains with confirmed AMR genes. Testing of 7857 clinical samples identified 425 Gram-negative bacteria out of which 82 appeared carbapenem resistant. These isolates were analyzed using VITEK-2 for phenotyping and the described AMR assay for genotyping. Results: The most prevalent carbapenemase gene was blaNDM and in 12 isolates we detected two carbapenemase genes (e.g., blaNDM/blaOXA-48). Conclusion: Our prevalence data from Pakistan show that-as in other parts of the world-carbapenemase-producing organisms with different underlying resistance mechanisms are emerging, and this warrants intensified and constant surveillance.",

keywords = "Pakistan",

author = "Braun, \{Sascha D.\} and Bushra Jamil and Syed, \{Muhammad A.\} and Abbasi, \{Shahid A.\} and Daniel Wei{\ss} and Peter Slickers and Stefan Monecke and Ines Engelmann and Ralf Ehricht",

note = "Publisher Copyright: {\textcopyright} 2018 Dr Sascha D Braun.",

year = "2018",

month = sep,

doi = "10.2217/fmb-2018-0082",

language = "English (UK)",

volume = "13",

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Braun, SD, Jamil, B, Syed, MA, Abbasi, SA, Weiß, D, Slickers, P, Monecke, S, Engelmann, I & Ehricht, R 2018, 'Prevalence of carbapenemase-producing organisms at the Kidney Center of Rawalpindi (Pakistan) and evaluation of an advanced molecular microarray-based carbapenemase assay', Future Microbiology, vol. 13, no. 11, pp. 1225-1246. https://doi.org/10.2217/fmb-2018-0082

TY - JOUR

T1 - Prevalence of carbapenemase-producing organisms at the Kidney Center of Rawalpindi (Pakistan) and evaluation of an advanced molecular microarray-based carbapenemase assay

AU - Braun, Sascha D.

AU - Jamil, Bushra

AU - Syed, Muhammad A.

AU - Abbasi, Shahid A.

AU - Weiß, Daniel

AU - Slickers, Peter

AU - Monecke, Stefan

AU - Engelmann, Ines

AU - Ehricht, Ralf

PY - 2018/9

Y1 - 2018/9

N2 - Aim: A DNA microarray-based assay for the detection of antimicrobial resistance (AMR) genes was used to study carbapenemase-producing organisms at the Kidney Center of Rawalpindi, Pakistan. Methods: The evaluation of this assay was performed using 97 reference strains with confirmed AMR genes. Testing of 7857 clinical samples identified 425 Gram-negative bacteria out of which 82 appeared carbapenem resistant. These isolates were analyzed using VITEK-2 for phenotyping and the described AMR assay for genotyping. Results: The most prevalent carbapenemase gene was blaNDM and in 12 isolates we detected two carbapenemase genes (e.g., blaNDM/blaOXA-48). Conclusion: Our prevalence data from Pakistan show that-as in other parts of the world-carbapenemase-producing organisms with different underlying resistance mechanisms are emerging, and this warrants intensified and constant surveillance.

AB - Aim: A DNA microarray-based assay for the detection of antimicrobial resistance (AMR) genes was used to study carbapenemase-producing organisms at the Kidney Center of Rawalpindi, Pakistan. Methods: The evaluation of this assay was performed using 97 reference strains with confirmed AMR genes. Testing of 7857 clinical samples identified 425 Gram-negative bacteria out of which 82 appeared carbapenem resistant. These isolates were analyzed using VITEK-2 for phenotyping and the described AMR assay for genotyping. Results: The most prevalent carbapenemase gene was blaNDM and in 12 isolates we detected two carbapenemase genes (e.g., blaNDM/blaOXA-48). Conclusion: Our prevalence data from Pakistan show that-as in other parts of the world-carbapenemase-producing organisms with different underlying resistance mechanisms are emerging, and this warrants intensified and constant surveillance.

KW - Pakistan

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DO - 10.2217/fmb-2018-0082

M3 - Article

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SN - 1746-0913

VL - 13

SP - 1225

EP - 1246

JO - Future Microbiology

JF - Future Microbiology

IS - 11

ER -

Prevalence of carbapenemase-producing organisms at the Kidney Center of Rawalpindi (Pakistan) and evaluation of an advanced molecular microarray-based carbapenemase assay

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