TY - JOUR
T1 - Protamine 1/Protamine 2 mRNA ratio in nonobstructive azoospermic patients
AU - Amjad, Sofia
AU - Mushtaq, Shamim
AU - Rehman, Rehana
AU - Munir, Adnan
AU - Zahid, Nida
AU - Siddique, Pirzada Qasim Raza
N1 - Funding Information:
The authors kindly thank participants of the study for their contribution. We are also thankful to Mussarat Ashraf from department of Biological & Biomedical Sciences, Aga Khan University, for assistance in our research work.
Publisher Copyright:
© 2021 Wiley-VCH GmbH
PY - 2021/4
Y1 - 2021/4
N2 - Altered protamine 1 (PRM1)/ protamine 2 (PRM2) mRNA ratio in testicular biopsy samples correlates with sperm quality and its fertilising ability. This study is planned to assess PRM1/ PRM2 mRNA ratio in subgroups of azoospermia to suggest a more reliable and accurate marker for assessing sperm quality in nonobstructive azoospermia (NOA). A cross-sectional study was done on testicular biopsy samples, taken from 106 azoospermic patients. Samples were histologically classified into subgroups: 36 obstructive azoospermia (OA), and two groups of NOA: 41 round spermatid maturation arrest (SMA) and 29 Sertoli cell-only syndrome (SCOS). OA samples showed histologically normal spermatogenesis and serve as a positive control. mRNA expression of jumonji domain-containing 1A (JMJD1A), PRM1, PRM2 and transition nuclear proteins (TNP1, TNP2) genes was determined, by RT-qPCR. Significantly lower expression of JMJD1A (p <.001), PRM1 (p =.0265) and PRM2 (p =.0032) has been seen in the SCOS group of NOA. We found significant (p <.001) increase in PRM1/PRM2 mRNA ratio in testicular biopsy samples of SCOS group of NOA patients and significant negative correlation of PRM1/PRM2 mRNA ratio with JMJD1A. Hence, PRM1/PRM2 mRNA ratio may represent a more reliable and accurate marker to assess sperm quality in NOA in addition to standard semen parameters.
AB - Altered protamine 1 (PRM1)/ protamine 2 (PRM2) mRNA ratio in testicular biopsy samples correlates with sperm quality and its fertilising ability. This study is planned to assess PRM1/ PRM2 mRNA ratio in subgroups of azoospermia to suggest a more reliable and accurate marker for assessing sperm quality in nonobstructive azoospermia (NOA). A cross-sectional study was done on testicular biopsy samples, taken from 106 azoospermic patients. Samples were histologically classified into subgroups: 36 obstructive azoospermia (OA), and two groups of NOA: 41 round spermatid maturation arrest (SMA) and 29 Sertoli cell-only syndrome (SCOS). OA samples showed histologically normal spermatogenesis and serve as a positive control. mRNA expression of jumonji domain-containing 1A (JMJD1A), PRM1, PRM2 and transition nuclear proteins (TNP1, TNP2) genes was determined, by RT-qPCR. Significantly lower expression of JMJD1A (p <.001), PRM1 (p =.0265) and PRM2 (p =.0032) has been seen in the SCOS group of NOA. We found significant (p <.001) increase in PRM1/PRM2 mRNA ratio in testicular biopsy samples of SCOS group of NOA patients and significant negative correlation of PRM1/PRM2 mRNA ratio with JMJD1A. Hence, PRM1/PRM2 mRNA ratio may represent a more reliable and accurate marker to assess sperm quality in NOA in addition to standard semen parameters.
KW - Sertoli cell-only syndrome
KW - azoospermia
KW - nonobstructive azoospermia
KW - protamine 1/protamine 2 mRNA ratio
KW - round spermatid maturation arrest
UR - http://www.scopus.com/inward/record.url?scp=85099087612&partnerID=8YFLogxK
U2 - 10.1111/and.13936
DO - 10.1111/and.13936
M3 - Article
C2 - 33427330
AN - SCOPUS:85099087612
SN - 0303-4569
VL - 53
JO - Andrologia
JF - Andrologia
IS - 3
M1 - e13936
ER -