Quantitative analysis of dopamine receptor messages in the mouse cochlea

Ahmad A. Karadaghy, John M. Lasak, Joseph S. Chomchai, Khalid M. Khan, Marian J. Drescher, Dennis G. Drescher

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)


Dopamine receptor isoforms were examined in the cochlea of the CBA(J) mouse by RT-PCR analysis and nucleotide sequencing, utilizing primers specific for known dopamine receptor isoforms. Cochlear cDNA sequences corresponding to dopamine D(2(long)) and D3 receptors were amplified, whereas those representing D(1A), D(1B), D(2(short)), and D4 were not detected. Utilizing quantitative competitive PCR analysis, relative levels of dopamine receptor transcripts were found to be 0.002, 0.014, 0.016, and 1.000 for D(2(long)) cochlea, D3 cochlea, D3 brain, and D(2(long)) brain, respectively. In the context of previously published findings, the current work provides key quantitative evidence necessary to establish that dopamine is a neurotransmitter in the auditory inner ear.

Original languageEnglish
Pages (from-to)151-156
Number of pages6
JournalMolecular Brain Research
Issue number1
Publication statusPublished - Feb 1997


  • Cochlea
  • Dopamine
  • Dopamine receptors
  • Neurotransmission
  • Quantitative competitive PCR


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