Screen printed carbon electrode based electrochemical immunosensor for the detection of dengue NS1 antigen

Om Parkash, Chan Yean Yean, Rafidah Hanim Shueb

Research output: Contribution to journalArticlepeer-review

46 Citations (Scopus)

Abstract

An electrochemical immunosensor modified with the streptavidin/biotin system on screen printed carbon electrodes (SPCEs) for the detection of the dengue NS1 antigen was developed in this study. Monoclonal anti-NS1 capture antibody was immobilized on streptavidin-modified SPCEs to increase the sensitivity of the assay. Subsequently, a direct sandwich enzyme linked immunosorbent assay (ELISA) format was developed and optimized. An anti-NS1 detection antibody conjugated with horseradish peroxidase enzyme (HRP) and 3,3,5,5'-tetramethybezidine dihydrochloride (TMB/H2O2) was used as an enzyme mediator. Electrochemical detection was conducted using the chronoamperometric technique, and electrochemical responses were generated at -200 mV reduction potential. The calibration curve of the immunosensor showed a linear response between 0.5 μg/mL and 2 μg/mL and a detection limit of 0.03 μg/mL. Incorporation of a streptavidin/biotin system resulted in a well-oriented antibody immobilization of the capture antibody and consequently enhanced the sensitivity of the assay. In conclusion, this immunosensor is a promising technology for the rapid and convenient detection of acute dengue infection in real serum samples.

Original languageEnglish
Pages (from-to)165-180
Number of pages16
JournalDiagnostics
Volume4
Issue number4
DOIs
Publication statusPublished - 2014
Externally publishedYes

Keywords

  • Dengue diagnosis
  • Electrochemical immunosensor
  • NS1
  • Screen printed carbon electrode
  • Streptavidin/biotin

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