Abstract
Seed protein profiling is the most promising tool in determining the molecular polymorphism and genetic homology. Seed storage proteins help in cultivar identification by avoiding the external environmental influences. Electrophoretically detectable proteins in rice grains possess the potential of characterizing the germplasms by their taxonomic and evolutionary aspects. This study was aimed at exploiting the genetic variations among ten elite rice genotypes of Sindh through electrophoretical separation of grain proteins by sodium dodecyl sulphate polyacryamide gel electrophoresis (SDSPAGE). In conclusion, the investigation revealed negligible polymorphism, with reference to the total seed protein profiles, among the rice genotypes of Sindh, Pakistan used in this study. Hence, it is highly important to include a significant number of rice genotypes to explore their existing genotypic diversity for future rice breeding programs. The SDS-PAGE in combination with 2-D electrophoresis is further suggested for documenting contrasting variations of isoforms of protein peptides.
| Original language | English |
|---|---|
| Pages (from-to) | 1106-1111 |
| Number of pages | 6 |
| Journal | African Journal of Biotechnology |
| Volume | 10 |
| Issue number | 7 |
| Publication status | Published - 14 Feb 2011 |
| Externally published | Yes |
Keywords
- Oryza sativa
- Polymorphism
- SDS-PAGE
- Seed storage proteins
- Sindh