Seed storage protein polymorphism in ten elite rice (Oryza sativa L.) genotypes of sindh

S. Galani, F. Naz, F. Soomro, I. Jamil, Zia-ul-hassan, A. Azhar, A. Ashraf

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

Seed protein profiling is the most promising tool in determining the molecular polymorphism and genetic homology. Seed storage proteins help in cultivar identification by avoiding the external environmental influences. Electrophoretically detectable proteins in rice grains possess the potential of characterizing the germplasms by their taxonomic and evolutionary aspects. This study was aimed at exploiting the genetic variations among ten elite rice genotypes of Sindh through electrophoretical separation of grain proteins by sodium dodecyl sulphate polyacryamide gel electrophoresis (SDSPAGE). In conclusion, the investigation revealed negligible polymorphism, with reference to the total seed protein profiles, among the rice genotypes of Sindh, Pakistan used in this study. Hence, it is highly important to include a significant number of rice genotypes to explore their existing genotypic diversity for future rice breeding programs. The SDS-PAGE in combination with 2-D electrophoresis is further suggested for documenting contrasting variations of isoforms of protein peptides.

Original languageEnglish
Pages (from-to)1106-1111
Number of pages6
JournalAfrican Journal of Biotechnology
Volume10
Issue number7
Publication statusPublished - 14 Feb 2011
Externally publishedYes

Keywords

  • Oryza sativa
  • Polymorphism
  • SDS-PAGE
  • Seed storage proteins
  • Sindh

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