TY - JOUR
T1 - Sentinel hospital-based surveillance for assessment of burden of rotavirus gastroenteritis in children in Pakistan
AU - Kazi, Abdul Momin
AU - Khan, Muhammad Mubashir Ahmad
AU - Zaidi, Anita Kaniz Mehdi
AU - Akhter, Rehana
AU - Aziz, Fatima
AU - Billoo, Abdul Gaffar
AU - Hafiz, Rehan
AU - Hussain, Ali Shabbir
AU - Ishaque, Muhammad
AU - Kabir, Furqan
AU - Kazmi, Shahzad
AU - Khattak, Afshan
AU - Maqbool, Sajid
AU - Memon, Ashraf
AU - Qazi, Yasmeen
AU - Rais, Abida
AU - Ramzan, Afroze
AU - Tariq, Parveen Akhter
AU - Un-Nisa, Tayyab
AU - Qureshi, Huma
AU - Qureshi, Shahida
AU - Warraich, Gohar Javed
N1 - Publisher Copyright:
© 2014 Raghunathan et al.
PY - 2014/10/8
Y1 - 2014/10/8
N2 - Objectives: To determine the burden and molecular epidemiology of rotavirus gastroenteritis in children hospitalized with severe acute watery diarrhea in Pakistan prior to introduction of rotavirus vaccine.Methods: A cross-sectional study was carried out over a period of two years from 2006-2008 at five sentinel hospitals in the cities of Karachi, Lahore, Rawalpindi, and Peshawar. Stool samples collected from children under five years of age hospitalized with severe acute watery diarrhea were tested for rotavirus antigen via enzyme immunoassay (EIA) (IDEA REF K6020 Oxoid Ltd (Ely), Cambridge, United Kingdom). A subset of EIA positive stool samples were further processed for genotyping.Results: 6679 children were enrolled and stool specimens of 2039 (30.5%) were positive for rotavirus. Rotavirus positivity ranged from 16.3% to 39.4% in the 5 hospitals with highest positivity in Lahore. 1241 (61%) of all rotavirus cases were in infants under one year of age. Among the strains examined for G-serotypes, the occurrence of G1, G2, G9 and G4 strains was found to be 28%, 24%, 14% and 13%, respectively. Among P-types, the most commonly occurring strains were P6 (31.5%) followed by P8 (20%) and P4 (12%). Prevalent rotavirus genotype in hospitalized children of severe diarrhea were G1P[8] 11.6% (69/593), followed by G2P[4] 10.4% (62/593), and G4P[6] 10.1% (60/593).Conclusions: Approximately one third of children hospitalized with severe gastroenteritis in urban centers in Pakistan have rotavirus. Introduction of rotavirus vaccine in Pakistan's national immunization program could prevent many severe episodes and diarrheal deaths.
AB - Objectives: To determine the burden and molecular epidemiology of rotavirus gastroenteritis in children hospitalized with severe acute watery diarrhea in Pakistan prior to introduction of rotavirus vaccine.Methods: A cross-sectional study was carried out over a period of two years from 2006-2008 at five sentinel hospitals in the cities of Karachi, Lahore, Rawalpindi, and Peshawar. Stool samples collected from children under five years of age hospitalized with severe acute watery diarrhea were tested for rotavirus antigen via enzyme immunoassay (EIA) (IDEA REF K6020 Oxoid Ltd (Ely), Cambridge, United Kingdom). A subset of EIA positive stool samples were further processed for genotyping.Results: 6679 children were enrolled and stool specimens of 2039 (30.5%) were positive for rotavirus. Rotavirus positivity ranged from 16.3% to 39.4% in the 5 hospitals with highest positivity in Lahore. 1241 (61%) of all rotavirus cases were in infants under one year of age. Among the strains examined for G-serotypes, the occurrence of G1, G2, G9 and G4 strains was found to be 28%, 24%, 14% and 13%, respectively. Among P-types, the most commonly occurring strains were P6 (31.5%) followed by P8 (20%) and P4 (12%). Prevalent rotavirus genotype in hospitalized children of severe diarrhea were G1P[8] 11.6% (69/593), followed by G2P[4] 10.4% (62/593), and G4P[6] 10.1% (60/593).Conclusions: Approximately one third of children hospitalized with severe gastroenteritis in urban centers in Pakistan have rotavirus. Introduction of rotavirus vaccine in Pakistan's national immunization program could prevent many severe episodes and diarrheal deaths.
UR - http://www.scopus.com/inward/record.url?scp=84907855641&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0108221
DO - 10.1371/journal.pone.0108221
M3 - Article
C2 - 25295613
AN - SCOPUS:84907855641
SN - 1932-6203
VL - 9
JO - PLoS ONE
JF - PLoS ONE
IS - 10
M1 - e108221
ER -