Size heterogeneity among antigenically related Giardia lamblia variant- specific surface proteins is due to differences in tandem repeat copy number

M. R. Mowatt; B. Y.T. Nguyen; J. T. Conrad; R. D. Adam; T. E. Nash

Size heterogeneity among antigenically related Giardia lamblia variant- specific surface proteins is due to differences in tandem repeat copy number

M. R. Mowatt, B. Y.T. Nguyen, J. T. Conrad, R. D. Adam, T. E. Nash

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

Giardia lamblia undergoes antigenic variation by modulating the expression of the different genes that comprise the trophozoite's variant-specific surface protein (VSP) repertoire. We studied an epitope that is conserved among VSPs expressed by cloned trophozoite lines derived from the independent G. lamblia isolates WB, G3M, Be-2, and CAT. The epitope recognized by monoclonal antibody 6E7 lies entirely within the region of tandemly repeated 65-amino-acid units that is characteristic of these size-variant VSPs. Northern (RNA) hybridization, cDNA cloning, and DNA sequence analysis indicate that size heterogeneity among these VSPs is due to differences in the number of repetitive units.

Original language	English
Pages (from-to)	1213-1218
Number of pages	6
Journal	Infection and Immunity
Volume	62
Issue number	4
Publication status	Published - 1994
Externally published	Yes

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title = "Size heterogeneity among antigenically related Giardia lamblia variant- specific surface proteins is due to differences in tandem repeat copy number",

abstract = "Giardia lamblia undergoes antigenic variation by modulating the expression of the different genes that comprise the trophozoite's variant-specific surface protein (VSP) repertoire. We studied an epitope that is conserved among VSPs expressed by cloned trophozoite lines derived from the independent G. lamblia isolates WB, G3M, Be-2, and CAT. The epitope recognized by monoclonal antibody 6E7 lies entirely within the region of tandemly repeated 65-amino-acid units that is characteristic of these size-variant VSPs. Northern (RNA) hybridization, cDNA cloning, and DNA sequence analysis indicate that size heterogeneity among these VSPs is due to differences in the number of repetitive units.",

author = "Mowatt, {M. R.} and Nguyen, {B. Y.T.} and Conrad, {J. T.} and Adam, {R. D.} and Nash, {T. E.}",

year = "1994",

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T1 - Size heterogeneity among antigenically related Giardia lamblia variant- specific surface proteins is due to differences in tandem repeat copy number

AU - Mowatt, M. R.

AU - Nguyen, B. Y.T.

AU - Conrad, J. T.

AU - Adam, R. D.

AU - Nash, T. E.

PY - 1994

Y1 - 1994

N2 - Giardia lamblia undergoes antigenic variation by modulating the expression of the different genes that comprise the trophozoite's variant-specific surface protein (VSP) repertoire. We studied an epitope that is conserved among VSPs expressed by cloned trophozoite lines derived from the independent G. lamblia isolates WB, G3M, Be-2, and CAT. The epitope recognized by monoclonal antibody 6E7 lies entirely within the region of tandemly repeated 65-amino-acid units that is characteristic of these size-variant VSPs. Northern (RNA) hybridization, cDNA cloning, and DNA sequence analysis indicate that size heterogeneity among these VSPs is due to differences in the number of repetitive units.

AB - Giardia lamblia undergoes antigenic variation by modulating the expression of the different genes that comprise the trophozoite's variant-specific surface protein (VSP) repertoire. We studied an epitope that is conserved among VSPs expressed by cloned trophozoite lines derived from the independent G. lamblia isolates WB, G3M, Be-2, and CAT. The epitope recognized by monoclonal antibody 6E7 lies entirely within the region of tandemly repeated 65-amino-acid units that is characteristic of these size-variant VSPs. Northern (RNA) hybridization, cDNA cloning, and DNA sequence analysis indicate that size heterogeneity among these VSPs is due to differences in the number of repetitive units.

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C2 - 7510666

AN - SCOPUS:0028345499

SN - 0019-9567

VL - 62

SP - 1213

EP - 1218

JO - Infection and Immunity

JF - Infection and Immunity

IS - 4

ER -

Size heterogeneity among antigenically related Giardia lamblia variant- specific surface proteins is due to differences in tandem repeat copy number

Abstract

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