TY - JOUR
T1 - Strong correspondence in bacterial loads between the vagina and rectum of pregnant women
AU - El Aila, Nabil Abdullah
AU - Tency, Inge
AU - Saerens, Bart
AU - De Backer, Ellen
AU - Cools, Piet
AU - dos Santos Santiago, Guido Lopes
AU - Verstraelen, Hans
AU - Verhelst, Rita
AU - Temmerman, Marleen
AU - Vaneechoutte, Mario
N1 - Funding Information:
Nabil Abdullah El Aila is indebted to the BOF-DOS of the University of Ghent-Belgium for PhD research funding; the study was further funded by the Concerted Research Actions Program (GOA) of the University of Ghent. BOF-DOS and GOA were not involved in development of the study design, collection, analysis or interpretation of data, nor in the writing of the report or the decision to submit the paper for publication.
PY - 2011/6
Y1 - 2011/6
N2 - We sampled the vagina and rectum in 71 pregnant women and bacterial loads of Lactobacillus crispatus, L. jensenii, L. gasseri, L. iners, Gardnerella vaginalis and Atopobium vaginae were determined by culture and quantitative PCR (qPCR).Culture and qPCR results differed substantially with regard to the evaluation of vaginal and rectal occurrence of the six species tested. The vaginal-rectal prevalence of L. crispatus, L. jensenii, L. gasseri, L. iners, G. vaginalis and A. vaginae as established by culture vs. PCR was 32.3 vs. 91.5%, 32.3 vs. 77.4%, 28.1 vs. 91.5%, 12.6 vs. 68.5%, 12.6 vs. 74.6% and 5.6 vs. 69.0%, respectively. Using qPCR, a significant positive correlation was found between vaginal and rectal loads of L. crispatus (p < 0.0001), L. jensenii (p < 0.0001), L. gasseri (p = 0.005), L. iners (p = 0.003) and A. vaginae (p = 0.002).In summary, significant correlations between quantities of vaginal and rectal lactobacilli and of Atopobium vaginae were established by means of qPCR, indicating strong correspondence of vaginal and rectal microflora, not only in the occurrence of certain species in both niches, but also of cell densities per bacterial species.
AB - We sampled the vagina and rectum in 71 pregnant women and bacterial loads of Lactobacillus crispatus, L. jensenii, L. gasseri, L. iners, Gardnerella vaginalis and Atopobium vaginae were determined by culture and quantitative PCR (qPCR).Culture and qPCR results differed substantially with regard to the evaluation of vaginal and rectal occurrence of the six species tested. The vaginal-rectal prevalence of L. crispatus, L. jensenii, L. gasseri, L. iners, G. vaginalis and A. vaginae as established by culture vs. PCR was 32.3 vs. 91.5%, 32.3 vs. 77.4%, 28.1 vs. 91.5%, 12.6 vs. 68.5%, 12.6 vs. 74.6% and 5.6 vs. 69.0%, respectively. Using qPCR, a significant positive correlation was found between vaginal and rectal loads of L. crispatus (p < 0.0001), L. jensenii (p < 0.0001), L. gasseri (p = 0.005), L. iners (p = 0.003) and A. vaginae (p = 0.002).In summary, significant correlations between quantities of vaginal and rectal lactobacilli and of Atopobium vaginae were established by means of qPCR, indicating strong correspondence of vaginal and rectal microflora, not only in the occurrence of certain species in both niches, but also of cell densities per bacterial species.
KW - Atopobium vaginae
KW - Gardnerella vaginalis
KW - Lactobacilli
KW - Pregnant women
KW - QPCR
UR - http://www.scopus.com/inward/record.url?scp=79957799750&partnerID=8YFLogxK
U2 - 10.1016/j.resmic.2011.04.004
DO - 10.1016/j.resmic.2011.04.004
M3 - Article
C2 - 21513795
AN - SCOPUS:79957799750
SN - 0923-2508
VL - 162
SP - 506
EP - 513
JO - Research in Microbiology
JF - Research in Microbiology
IS - 5
ER -