TY - JOUR
T1 - Studies on anti-inflammatory and analgesic activities of betel nut in rodents
AU - Khan, Shagufta
AU - Mehmood, Malik Hassan
AU - Ali, Anita Naushir Akbar
AU - Ahmed, Fahad Shabbir
AU - Dar, Ahsana
AU - Gilani, Anwarul Hassan
PY - 2011/6/1
Y1 - 2011/6/1
N2 - Ethnopharmacological relevance: Areca catechu, commonly known as betel nut, is very famous for its medicinal use in multiple disorders. It is also popular as a remedy against inflammatory disorders in the Unani (Greco-Arab) system of medicine. Objective of the study: This study was aimed at investigating the anti-inflammatory and analgesic activities of the crude extract of Areca catechu and its respective fractions. Materials and methods: Paw edema, formalin-induced nociception and acetic acid-induced writhing assays were carried out in vivo. Free radical scavenging activity of the plant extract was performed in vitro. Results: Preliminary experiments using a single dose (100 mg/kg) of Areca catechu and its respective fractions demonstrated an anti-inflammatory effect on carrageenan-induced edema in mice and rats, the aqueous fraction being distinctly more effective. When studied on prostaglandin E 2 (PGE 2), arachidonic acid, histamine, or serotonin (5HT)-induced edema in rats, Areca catechu and its aqueous fraction markedly repressed only the PGE 2 and arachidonic acid-induced inflammation. When studied for analgesic activity, the crude extract and its aqueous fraction produced a dose-dependent (10-100 mg/kg) inhibitory effect on formalin-induced nociception in mice and acetic acid-induced writhing in rats, similar to aspirin. In DPPH assay, Areca catechu and its aqueous fraction exhibited free radical scavenging activity with respective IC 50 values of 5.34 μg/ml (4.93-5.78, CI; 95%, n = 5) and 7.28 μg/ml (6.04-7.95, n = 4), like that of rutin with IC 50 value of 4.75 μg/ml (3.89-5.42, n = 4). Conclusion: These results indicate the anti-inflammatory and analgesic effects of Areca catechu and provide a rationale for its medicinal use in inflammatory disorders.
AB - Ethnopharmacological relevance: Areca catechu, commonly known as betel nut, is very famous for its medicinal use in multiple disorders. It is also popular as a remedy against inflammatory disorders in the Unani (Greco-Arab) system of medicine. Objective of the study: This study was aimed at investigating the anti-inflammatory and analgesic activities of the crude extract of Areca catechu and its respective fractions. Materials and methods: Paw edema, formalin-induced nociception and acetic acid-induced writhing assays were carried out in vivo. Free radical scavenging activity of the plant extract was performed in vitro. Results: Preliminary experiments using a single dose (100 mg/kg) of Areca catechu and its respective fractions demonstrated an anti-inflammatory effect on carrageenan-induced edema in mice and rats, the aqueous fraction being distinctly more effective. When studied on prostaglandin E 2 (PGE 2), arachidonic acid, histamine, or serotonin (5HT)-induced edema in rats, Areca catechu and its aqueous fraction markedly repressed only the PGE 2 and arachidonic acid-induced inflammation. When studied for analgesic activity, the crude extract and its aqueous fraction produced a dose-dependent (10-100 mg/kg) inhibitory effect on formalin-induced nociception in mice and acetic acid-induced writhing in rats, similar to aspirin. In DPPH assay, Areca catechu and its aqueous fraction exhibited free radical scavenging activity with respective IC 50 values of 5.34 μg/ml (4.93-5.78, CI; 95%, n = 5) and 7.28 μg/ml (6.04-7.95, n = 4), like that of rutin with IC 50 value of 4.75 μg/ml (3.89-5.42, n = 4). Conclusion: These results indicate the anti-inflammatory and analgesic effects of Areca catechu and provide a rationale for its medicinal use in inflammatory disorders.
KW - Analgesic
KW - Anti-inflammatory
KW - Anti-oxidant
KW - Betel nut
KW - Cyclooxygenase inhibitor
KW - Lipoxygenase inhibitor
UR - http://www.scopus.com/inward/record.url?scp=79957464125&partnerID=8YFLogxK
U2 - 10.1016/j.jep.2011.03.064
DO - 10.1016/j.jep.2011.03.064
M3 - Article
C2 - 21501676
AN - SCOPUS:79957464125
SN - 0378-8741
VL - 135
SP - 654
EP - 661
JO - Journal of Ethnopharmacology
JF - Journal of Ethnopharmacology
IS - 3
ER -