The preparation of 188Re-glucoheptonate (GH) is described using 188Re, which was obtained from an alumina based 188W/188Re generator. The dependence of the radiolabeling yields of 188Re-GH on reducing agent concentration, GH concentration, pH, temperature and incubation time was examined. In the case of optimum conditions the yield of 188Re-GH was ∼99%. The ITLC technique was employed to monitor the different species formed. A biodistribution study of 188Re-GH was carried out in rats and compared to the biological behavior of 99mTc-GH.