T-cell recognition of the 18-kilodalton antigen of Mycobacterium leprae

H. M. Dockrell; N. G. Stoker; S. P. Lee; M. Jackson; K. A. Grant; N. F. Jouy; S. B. Lucas; R. Hasan; R. Hussain; K. P.W.J. McAdam

T-cell recognition of the 18-kilodalton antigen of Mycobacterium leprae

H. M. Dockrell, N. G. Stoker, S. P. Lee, M. Jackson, K. A. Grant, N. F. Jouy, S. B. Lucas, R. Hasan, R. Hussain, K. P.W.J. McAdam

Research output: Contribution to journal › Article › peer-review

45 Citations (Scopus)

Abstract

The 18-kilodalton (kDa) antigen of Mycobacterium leprae was expressed as a fusion protein with a 2-kDa leader peptide and used in proliferation assays with peripheral blood cells. Fifty percent of untreated tuberculoid leprosy patients and 93% of long-term leprosy contacts responded to the recombinant protein in lymphocyte transformation tests. Comparison of the stimulation indices in the two groups showed that the contacts responded more strongly than the tuberculoid leprosy patients. Seventy percent of Myobacterium bovis BCG-vaccinated European donors responded, although with low stimulation indices. The isolation of 18-kDa antigen-responsive T-cell lines from a BCG-vaccinated British donor confirmed that the 18-kDa antigen contains at least one cross-reactive epitope. These results indicate that the 18-kDa protein is an important antigen in the immune response to leprosy.

Original language	English
Pages (from-to)	1979-1983
Number of pages	5
Journal	Infection and Immunity
Volume	57
Issue number	7
Publication status	Published - 1989
Externally published	Yes

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title = "T-cell recognition of the 18-kilodalton antigen of Mycobacterium leprae",

abstract = "The 18-kilodalton (kDa) antigen of Mycobacterium leprae was expressed as a fusion protein with a 2-kDa leader peptide and used in proliferation assays with peripheral blood cells. Fifty percent of untreated tuberculoid leprosy patients and 93% of long-term leprosy contacts responded to the recombinant protein in lymphocyte transformation tests. Comparison of the stimulation indices in the two groups showed that the contacts responded more strongly than the tuberculoid leprosy patients. Seventy percent of Myobacterium bovis BCG-vaccinated European donors responded, although with low stimulation indices. The isolation of 18-kDa antigen-responsive T-cell lines from a BCG-vaccinated British donor confirmed that the 18-kDa antigen contains at least one cross-reactive epitope. These results indicate that the 18-kDa protein is an important antigen in the immune response to leprosy.",

author = "Dockrell, {H. M.} and Stoker, {N. G.} and Lee, {S. P.} and M. Jackson and Grant, {K. A.} and Jouy, {N. F.} and Lucas, {S. B.} and R. Hasan and R. Hussain and McAdam, {K. P.W.J.}",

year = "1989",

language = "English",

volume = "57",

pages = "1979--1983",

journal = "Infection and Immunity",

issn = "0019-9567",

publisher = "American Society for Microbiology",

number = "7",

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TY - JOUR

T1 - T-cell recognition of the 18-kilodalton antigen of Mycobacterium leprae

AU - Dockrell, H. M.

AU - Stoker, N. G.

AU - Lee, S. P.

AU - Jackson, M.

AU - Grant, K. A.

AU - Jouy, N. F.

AU - Lucas, S. B.

AU - Hasan, R.

AU - Hussain, R.

AU - McAdam, K. P.W.J.

PY - 1989

Y1 - 1989

N2 - The 18-kilodalton (kDa) antigen of Mycobacterium leprae was expressed as a fusion protein with a 2-kDa leader peptide and used in proliferation assays with peripheral blood cells. Fifty percent of untreated tuberculoid leprosy patients and 93% of long-term leprosy contacts responded to the recombinant protein in lymphocyte transformation tests. Comparison of the stimulation indices in the two groups showed that the contacts responded more strongly than the tuberculoid leprosy patients. Seventy percent of Myobacterium bovis BCG-vaccinated European donors responded, although with low stimulation indices. The isolation of 18-kDa antigen-responsive T-cell lines from a BCG-vaccinated British donor confirmed that the 18-kDa antigen contains at least one cross-reactive epitope. These results indicate that the 18-kDa protein is an important antigen in the immune response to leprosy.

AB - The 18-kilodalton (kDa) antigen of Mycobacterium leprae was expressed as a fusion protein with a 2-kDa leader peptide and used in proliferation assays with peripheral blood cells. Fifty percent of untreated tuberculoid leprosy patients and 93% of long-term leprosy contacts responded to the recombinant protein in lymphocyte transformation tests. Comparison of the stimulation indices in the two groups showed that the contacts responded more strongly than the tuberculoid leprosy patients. Seventy percent of Myobacterium bovis BCG-vaccinated European donors responded, although with low stimulation indices. The isolation of 18-kDa antigen-responsive T-cell lines from a BCG-vaccinated British donor confirmed that the 18-kDa antigen contains at least one cross-reactive epitope. These results indicate that the 18-kDa protein is an important antigen in the immune response to leprosy.

UR - http://www.scopus.com/inward/record.url?scp=0024344687&partnerID=8YFLogxK

M3 - Article

C2 - 2659530

AN - SCOPUS:0024344687

SN - 0019-9567

VL - 57

SP - 1979

EP - 1983

JO - Infection and Immunity

JF - Infection and Immunity

IS - 7

ER -

T-cell recognition of the 18-kilodalton antigen of Mycobacterium leprae

Abstract

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