TY - JOUR
T1 - Technetium-99m direct radiolabeling of Lanreotide
T2 - A somatostatin analog
AU - Pervez, Shahid
AU - Mushtaq, A.
AU - Arif, Muhammad
N1 - Funding Information:
This work was sponsored by the International Atomic Energy Agency (IAEA), Vienna, under research contract No. 10107/RB.
PY - 2001
Y1 - 2001
N2 - Lanreotide, a synthetic octapeptide analog of a native hormone somatostatin, was labeled with a commonly available, inexpensive radionuclide 99mTc. Labeling was accomplished by reduction of the cysteine bridge, which provided sulfhydryl groups for chelation with 99mTc. Stannous chloride was used as reducing agent, while tartrate acted as transchelating agent. Lanreotide (100 μg), stannous chloride dihydrate (100 μg) and tartaric acid (64 μg) were dissolved in acetate/acetic acid buffer (pH 2.8). After overnight (∼ 18 h) incubation, ∼ 444 MBq (12 mCi) 99mTc was added and kept in boiling water for 30 min. More than 97% labeling efficiency was confirmed by RP-HPLC, ITLC-SG and C18 cartridge analysis. Radiolabeling results in one major peak when analyzed by reverse-phase HPLC. The stability of the 99mTc-peptide bond was evaluated by cysteine challenge studies.
AB - Lanreotide, a synthetic octapeptide analog of a native hormone somatostatin, was labeled with a commonly available, inexpensive radionuclide 99mTc. Labeling was accomplished by reduction of the cysteine bridge, which provided sulfhydryl groups for chelation with 99mTc. Stannous chloride was used as reducing agent, while tartrate acted as transchelating agent. Lanreotide (100 μg), stannous chloride dihydrate (100 μg) and tartaric acid (64 μg) were dissolved in acetate/acetic acid buffer (pH 2.8). After overnight (∼ 18 h) incubation, ∼ 444 MBq (12 mCi) 99mTc was added and kept in boiling water for 30 min. More than 97% labeling efficiency was confirmed by RP-HPLC, ITLC-SG and C18 cartridge analysis. Radiolabeling results in one major peak when analyzed by reverse-phase HPLC. The stability of the 99mTc-peptide bond was evaluated by cysteine challenge studies.
KW - Direct radiolabeling
KW - HPLC
KW - Peptide
KW - Technetium-99m
UR - http://www.scopus.com/inward/record.url?scp=0034888236&partnerID=8YFLogxK
U2 - 10.1016/S0969-8043(01)00118-X
DO - 10.1016/S0969-8043(01)00118-X
M3 - Article
C2 - 11573798
AN - SCOPUS:0034888236
SN - 0969-8043
VL - 55
SP - 647
EP - 651
JO - Applied Radiation and Isotopes
JF - Applied Radiation and Isotopes
IS - 5
ER -