TFF1 is membrane-associated in breast carcinoma cell line MCF-7

Lai San Siu; Hanna Romanska; Paul D. Abel; Tuncay Kayademir; Nikolaus Blin; Gordon W.H. Stamp; El Nasir Lalani

doi:10.1016/j.peptides.2004.01.024

TFF1 is membrane-associated in breast carcinoma cell line MCF-7

Lai San Siu, Hanna Romanska, Paul D. Abel, Tuncay Kayademir, Nikolaus Blin, Gordon W.H. Stamp, El Nasir Lalani

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Trefoil factor family (TFF) domain peptides, products of mucin-secreting epithelial cells, are thought to influence mucosal integrity. Molecular studies revealed that mammalian TFFs lack transmembrane domains. Using immunocytochemistry and FACS analysis we demonstrated the association of TFF1 with the cell membrane in MCF-7 (a breast adenocarcinoma cell line), and tested the hypothesis that glycosylphosphatidylinositol (GPI) linkage is the mechanism for this association. Cleavage of GPI anchorage using phospholipase C did not affect TFF1 binding to the cell membrane. Our results demonstrate for the first time that TFF1 is associated with the cell membrane of MCF-7 cells and is not linked via a GPI anchor.

Original language	English
Pages (from-to)	745-753
Number of pages	9
Journal	Peptides
Volume	25
Issue number	5
DOIs	https://doi.org/10.1016/j.peptides.2004.01.024
Publication status	Published - May 2004
Externally published	Yes

Keywords

Breast carcinoma
Glycosylphosphatidylinositol
TFF1

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10.1016/j.peptides.2004.01.024

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title = "TFF1 is membrane-associated in breast carcinoma cell line MCF-7",

abstract = "Trefoil factor family (TFF) domain peptides, products of mucin-secreting epithelial cells, are thought to influence mucosal integrity. Molecular studies revealed that mammalian TFFs lack transmembrane domains. Using immunocytochemistry and FACS analysis we demonstrated the association of TFF1 with the cell membrane in MCF-7 (a breast adenocarcinoma cell line), and tested the hypothesis that glycosylphosphatidylinositol (GPI) linkage is the mechanism for this association. Cleavage of GPI anchorage using phospholipase C did not affect TFF1 binding to the cell membrane. Our results demonstrate for the first time that TFF1 is associated with the cell membrane of MCF-7 cells and is not linked via a GPI anchor.",

keywords = "Breast carcinoma, Glycosylphosphatidylinositol, TFF1",

author = "Siu, {Lai San} and Hanna Romanska and Abel, {Paul D.} and Tuncay Kayademir and Nikolaus Blin and Stamp, {Gordon W.H.} and Lalani, {El Nasir}",

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language = "English",

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TY - JOUR

T1 - TFF1 is membrane-associated in breast carcinoma cell line MCF-7

AU - Siu, Lai San

AU - Romanska, Hanna

AU - Abel, Paul D.

AU - Kayademir, Tuncay

AU - Blin, Nikolaus

AU - Stamp, Gordon W.H.

AU - Lalani, El Nasir

PY - 2004/5

Y1 - 2004/5

N2 - Trefoil factor family (TFF) domain peptides, products of mucin-secreting epithelial cells, are thought to influence mucosal integrity. Molecular studies revealed that mammalian TFFs lack transmembrane domains. Using immunocytochemistry and FACS analysis we demonstrated the association of TFF1 with the cell membrane in MCF-7 (a breast adenocarcinoma cell line), and tested the hypothesis that glycosylphosphatidylinositol (GPI) linkage is the mechanism for this association. Cleavage of GPI anchorage using phospholipase C did not affect TFF1 binding to the cell membrane. Our results demonstrate for the first time that TFF1 is associated with the cell membrane of MCF-7 cells and is not linked via a GPI anchor.

AB - Trefoil factor family (TFF) domain peptides, products of mucin-secreting epithelial cells, are thought to influence mucosal integrity. Molecular studies revealed that mammalian TFFs lack transmembrane domains. Using immunocytochemistry and FACS analysis we demonstrated the association of TFF1 with the cell membrane in MCF-7 (a breast adenocarcinoma cell line), and tested the hypothesis that glycosylphosphatidylinositol (GPI) linkage is the mechanism for this association. Cleavage of GPI anchorage using phospholipase C did not affect TFF1 binding to the cell membrane. Our results demonstrate for the first time that TFF1 is associated with the cell membrane of MCF-7 cells and is not linked via a GPI anchor.

KW - Breast carcinoma

KW - Glycosylphosphatidylinositol

KW - TFF1

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U2 - 10.1016/j.peptides.2004.01.024

DO - 10.1016/j.peptides.2004.01.024

M3 - Article

C2 - 15177868

AN - SCOPUS:2642557775

SN - 0196-9781

VL - 25

SP - 745

EP - 753

JO - Peptides

JF - Peptides

IS - 5

ER -

TFF1 is membrane-associated in breast carcinoma cell line MCF-7

Abstract

Keywords

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