TY - JOUR
T1 - Th1/Th2 Cytometric Bead Array can discriminate cytokine secretion from endogenously activated cells in pulmonary disease, recent and remote infection in tuberculosis
AU - Talat, Najeeha
AU - Shahid, Firdaus
AU - Perry, Sharon
AU - Dawood, Ghaffar
AU - Hussain, Rabia
N1 - Funding Information:
We acknowledge the Higher Education Commission (HEC), Government of Pakistan for providing financial support for this project through Grant No. 20-796/R&D/07 . We also thank Mr. Mohammed Anwar for blood collection, Sindhu Muzaffar for technical help with CBA analysis and Ms. Regina D’souza for secretarial help. We are also grateful to Ms. Maqboola Dojki for administrative and logistics support. We also thank Mr. Iqbal Azam for helping in ROC analysis and excellent technical support from Mr. Zubair Ahmad, representative of local distributor of BD Biosciences. We would also like to thank Ms. Shufang Yang and Ms. Luz Sanchez, University of Stanford, CA, for their administrative support.
PY - 2011/5
Y1 - 2011/5
N2 - Differential T cell trafficking through the blood compartment towards infected foci may be occurring in different stages of tuberculosis disease and infection. The aim of the present study was to identify cytokine signatures in the blood compartment in tuberculosis patients with pulmonary disease (PTB. = 19), recently exposed household contacts (HC. = 27) and nonexposed community controls (EC. = 37). Diluted (1:10) whole blood was cultured for 2. days and cytokine secretion was assessed using Cytometric Bead Array (Th1/Th2 kit II; BD Biosciences) which included IL-2, TNF-α, IFN-γ (Type1/T1), IL-4, IL-6 and IL-10 (Type2/T2). All T1/T2 cytokines were elevated in PTB (AUROC > 0.9) while HC showed selective elevation of IL-6 (AUROC > 0.7) compared to EC. Principal component analysis (PCA) extracted two groupings with Eigen values >1; IL-6 separated into the second component for PTB, HC and EC. After rotation, IFN-γ was correlated with the first component for PTB and EC and the second component for HC indicating an absence of T1/T2 dichotomy. Therefore endogenous cytokine signatures may indicate differential T cell trafficking in different stages of tuberculosis infection and disease.
AB - Differential T cell trafficking through the blood compartment towards infected foci may be occurring in different stages of tuberculosis disease and infection. The aim of the present study was to identify cytokine signatures in the blood compartment in tuberculosis patients with pulmonary disease (PTB. = 19), recently exposed household contacts (HC. = 27) and nonexposed community controls (EC. = 37). Diluted (1:10) whole blood was cultured for 2. days and cytokine secretion was assessed using Cytometric Bead Array (Th1/Th2 kit II; BD Biosciences) which included IL-2, TNF-α, IFN-γ (Type1/T1), IL-4, IL-6 and IL-10 (Type2/T2). All T1/T2 cytokines were elevated in PTB (AUROC > 0.9) while HC showed selective elevation of IL-6 (AUROC > 0.7) compared to EC. Principal component analysis (PCA) extracted two groupings with Eigen values >1; IL-6 separated into the second component for PTB, HC and EC. After rotation, IFN-γ was correlated with the first component for PTB and EC and the second component for HC indicating an absence of T1/T2 dichotomy. Therefore endogenous cytokine signatures may indicate differential T cell trafficking in different stages of tuberculosis infection and disease.
KW - Cytometric Bead Array
KW - Tuberculosis
KW - Type1/Type2 cytokines
UR - http://www.scopus.com/inward/record.url?scp=79953043999&partnerID=8YFLogxK
U2 - 10.1016/j.cyto.2011.01.012
DO - 10.1016/j.cyto.2011.01.012
M3 - Article
C2 - 21315616
AN - SCOPUS:79953043999
SN - 1043-4666
VL - 54
SP - 136
EP - 143
JO - Cytokine
JF - Cytokine
IS - 2
ER -
