TY - JOUR
T1 - Titanium dioxide-tetra sulphonatophenyl porphyrin nanocomposites for target cellular bio-imaging and treatment of rheumatoid arthritis
AU - Zhao, Chunqiu
AU - Rehman, Fawad Ur
AU - Jiang, Hui
AU - Selke, Matthias
AU - Wang, Xuemei
AU - Liu, Chong Yang
N1 - Publisher Copyright:
© 2016, Science China Press and Springer-Verlag Berlin Heidelberg.
PY - 2016/5/1
Y1 - 2016/5/1
N2 - Photodynamic therapy (PDT) is one of the latest biomedical technologies used for treatment of various neoplastic and non-neoplastic diseases. However, there still exist some well-known problems regarding its efficacy, e.g. effective concentration of the drug at the desired sites, the irradiation light dosimetry and biocompatibility of the photosensitizer. The introduction of nanotechnology and nanomaterial like biocompatible nano-titania (i.e., nano-TiO2) may facilitate to solve some of these problems. In this study we have explored the possibility of combining tetra sulphonatophenyl porphyrin (TSPP) with nano-titania (PT) for efficient PDT with least adverse effects. The spectroscopic properties of these nano-composites were characterized by using fluorescence and UV-Vis absorption spectroscopic study. The singlet oxygen quantum yield was determined by using 2,5-diphenyl-3,4-benzofuran (DPBF), while the effect of nano TiO2 with TSPP on the synovial fibroblast cells from human (HSC) and rat models (RSC) were investigated by confocal laser microscopy and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Our results suggest that nano TiO2 with TSPP can be readily utilized for effective PDT treatment of Rheumatoid Arthritis (RA).
AB - Photodynamic therapy (PDT) is one of the latest biomedical technologies used for treatment of various neoplastic and non-neoplastic diseases. However, there still exist some well-known problems regarding its efficacy, e.g. effective concentration of the drug at the desired sites, the irradiation light dosimetry and biocompatibility of the photosensitizer. The introduction of nanotechnology and nanomaterial like biocompatible nano-titania (i.e., nano-TiO2) may facilitate to solve some of these problems. In this study we have explored the possibility of combining tetra sulphonatophenyl porphyrin (TSPP) with nano-titania (PT) for efficient PDT with least adverse effects. The spectroscopic properties of these nano-composites were characterized by using fluorescence and UV-Vis absorption spectroscopic study. The singlet oxygen quantum yield was determined by using 2,5-diphenyl-3,4-benzofuran (DPBF), while the effect of nano TiO2 with TSPP on the synovial fibroblast cells from human (HSC) and rat models (RSC) were investigated by confocal laser microscopy and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Our results suggest that nano TiO2 with TSPP can be readily utilized for effective PDT treatment of Rheumatoid Arthritis (RA).
KW - photodynamic therapy
KW - synovial fibroblasts
KW - tetra sulphonatophenyl porphyrin
KW - TiO nanoparticles (P25)
UR - https://www.scopus.com/pages/publications/84959568957
U2 - 10.1007/s11426-016-5568-1
DO - 10.1007/s11426-016-5568-1
M3 - Article
AN - SCOPUS:84959568957
SN - 1674-7291
VL - 59
SP - 637
EP - 642
JO - Science China Chemistry
JF - Science China Chemistry
IS - 5
ER -
