Transcriptional control of TFF3 (intestinal trefoil factor) via promoter binding sites for the nuclear factor κB and C/EBPβ

Mirela Baus-Loncar; Ezzal Din Al-azzeh; Hanna Romanska; El Nasir Lalani; Gordon W.H. Stamp; Nikolaus Blin; Tuncay Kayademir

doi:10.1016/j.peptides.2003.11.019

Transcriptional control of TFF3 (intestinal trefoil factor) via promoter binding sites for the nuclear factor κB and C/EBPβ

Mirela Baus-Loncar, Ezzal Din Al-azzeh, Hanna Romanska, El Nasir Lalani, Gordon W.H. Stamp, Nikolaus Blin, Tuncay Kayademir

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

The acute phase response is strictly connected with modulation of gene expression. Transcriptional control of many genes is mediated by binding of diverse transcription factors to cis-acting DNA motifs in the respective promoter sequence. We now describe such regulatory elements for the TFF3 gene coding for a peptide involved in response to gut irritation. TNF-α stimulation, which induces NF-κB activation, evoked up to 10-fold reduction of TFF3 expression in the colon tumour cell line HT-29. Several consensus binding sites for members of the NF-κB transcription factor subunits are located in the 5′-flanking region of TFF3. Mutating these sites was aimed at discovering which one is responsible for NF-κB binding and thus regulation of TFF3 expression.

Original language	English
Pages (from-to)	849-854
Number of pages	6
Journal	Peptides
Volume	25
Issue number	5
DOIs	https://doi.org/10.1016/j.peptides.2003.11.019
Publication status	Published - May 2004
Externally published	Yes

Keywords

Expression control
Signalling pathway
Transcription factor NF-κB
Trefoil factor family TFF

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10.1016/j.peptides.2003.11.019

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title = "Transcriptional control of TFF3 (intestinal trefoil factor) via promoter binding sites for the nuclear factor κB and C/EBPβ",

abstract = "The acute phase response is strictly connected with modulation of gene expression. Transcriptional control of many genes is mediated by binding of diverse transcription factors to cis-acting DNA motifs in the respective promoter sequence. We now describe such regulatory elements for the TFF3 gene coding for a peptide involved in response to gut irritation. TNF-α stimulation, which induces NF-κB activation, evoked up to 10-fold reduction of TFF3 expression in the colon tumour cell line HT-29. Several consensus binding sites for members of the NF-κB transcription factor subunits are located in the 5′-flanking region of TFF3. Mutating these sites was aimed at discovering which one is responsible for NF-κB binding and thus regulation of TFF3 expression.",

keywords = "Expression control, Signalling pathway, Transcription factor NF-κB, Trefoil factor family TFF",

author = "Mirela Baus-Loncar and Al-azzeh, {Ezzal Din} and Hanna Romanska and Lalani, {El Nasir} and Stamp, {Gordon W.H.} and Nikolaus Blin and Tuncay Kayademir",

note = "Funding Information: Supported by the Croatian Ministry of Science and Engelhorn Stiftung to MB-L and TK. We thank P Fegert, M Broghammer, F Gebhardt and M Vaegler for excellent technical assistance.",

year = "2004",

month = may,

doi = "10.1016/j.peptides.2003.11.019",

language = "English",

volume = "25",

pages = "849--854",

journal = "Peptides",

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T1 - Transcriptional control of TFF3 (intestinal trefoil factor) via promoter binding sites for the nuclear factor κB and C/EBPβ

AU - Baus-Loncar, Mirela

AU - Al-azzeh, Ezzal Din

AU - Romanska, Hanna

AU - Lalani, El Nasir

AU - Stamp, Gordon W.H.

AU - Blin, Nikolaus

AU - Kayademir, Tuncay

N1 - Funding Information: Supported by the Croatian Ministry of Science and Engelhorn Stiftung to MB-L and TK. We thank P Fegert, M Broghammer, F Gebhardt and M Vaegler for excellent technical assistance.

PY - 2004/5

Y1 - 2004/5

N2 - The acute phase response is strictly connected with modulation of gene expression. Transcriptional control of many genes is mediated by binding of diverse transcription factors to cis-acting DNA motifs in the respective promoter sequence. We now describe such regulatory elements for the TFF3 gene coding for a peptide involved in response to gut irritation. TNF-α stimulation, which induces NF-κB activation, evoked up to 10-fold reduction of TFF3 expression in the colon tumour cell line HT-29. Several consensus binding sites for members of the NF-κB transcription factor subunits are located in the 5′-flanking region of TFF3. Mutating these sites was aimed at discovering which one is responsible for NF-κB binding and thus regulation of TFF3 expression.

AB - The acute phase response is strictly connected with modulation of gene expression. Transcriptional control of many genes is mediated by binding of diverse transcription factors to cis-acting DNA motifs in the respective promoter sequence. We now describe such regulatory elements for the TFF3 gene coding for a peptide involved in response to gut irritation. TNF-α stimulation, which induces NF-κB activation, evoked up to 10-fold reduction of TFF3 expression in the colon tumour cell line HT-29. Several consensus binding sites for members of the NF-κB transcription factor subunits are located in the 5′-flanking region of TFF3. Mutating these sites was aimed at discovering which one is responsible for NF-κB binding and thus regulation of TFF3 expression.

KW - Expression control

KW - Signalling pathway

KW - Transcription factor NF-κB

KW - Trefoil factor family TFF

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U2 - 10.1016/j.peptides.2003.11.019

DO - 10.1016/j.peptides.2003.11.019

M3 - Article

C2 - 15177881

AN - SCOPUS:2642567501

SN - 0196-9781

VL - 25

SP - 849

EP - 854

JO - Peptides

JF - Peptides

IS - 5

ER -

Transcriptional control of TFF3 (intestinal trefoil factor) via promoter binding sites for the nuclear factor κB and C/EBPβ

Abstract

Keywords

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