Use of p-nitrobenzoic acid in 7H10 agar for identification of Mycobacterium tuberculosis complex: A field study

S. Shakoor, T. Ahsan, Kauser Jabeen, M. Raza, R. Hasan

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8 Citations (Scopus)

Abstract

We report the efficiency and cost-effectiveness of p-nitrobenzoic acid (PNB) testing in Middlebrook 7H10 agar medium for the identification of Mycobacterium tuberculosis complex (MTC). PNB-7H10 was compared with PNB-MGIT and BACTEC-NAP using 200 clinical mycobacterial isolates. PNB-7H10 showed 100% agreement with PNB-MGIT and BACTEC-NAP tests, and reduced the cost of PNB-MGIT test by 80%. PNB-7H10 agar is therefore an effective alternative to the costly PNB-MGIT and BACTEC-NAP tests, especially in resource-poor settings.

Original languageEnglish
Pages (from-to)1644-1646
Number of pages3
JournalInternational Journal of Tuberculosis and Lung Disease
Volume14
Issue number12
Publication statusPublished - Dec 2010

Keywords

  • Middlebrook 7H10 agar
  • Mycobacterium tuberculosis
  • P-nitrobenzoic acid

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